4-Thio-UTP

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4-Thio-UTP

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

包装与价格：

包装	价格(元)
10ul (100 mM)	电议
50ul (100 mM)	电议
100ul (100 mM)	电议

产品介绍

化学性质

Physical Appearance	Solution
Storage	Store at -20°C or below
M.Wt	500.2 (free acid)
Formula	C₉H₁₅N₂O₁₄P₃S (free acid)
Synonyms	4-Thiouridine-5'-Triphosphate, Thio UTP
Chemical Name	lithium (3,4-dihydroxy-5-(2-oxo-4-thioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)methyl triphosphate
Canonical SMILES	S=C(C=CN1C2C(O)C(O)C(COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O2)NC1=O.[Li+].[Li+].[Li+].[Li+]
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

试验操作

In Vitro Experiments [1]
In vitro transcription assays and photoaffinity labelling of the transcriptional complex.
Methods	In vitro transcription reactions were carried out using RNA polymerase purified from lysed pea chloroplasts. The enzyme was incubated with the template DNA (5A/30T) containing the 16S rRNA promoter and a-32P-ATP (30 μCi, sp. act. 3000 Ci/mmole) and the photoprobe 4-thio UTP (200 μM) in the presence of 50mM Tris-HCl, pH 8.0, 10mM DTT, 1 μg BSA and 5mM MgCl2 at 25°C for 45 min.

生物活性

4-Thiouridine-5'-Triphosphate, also known as 4-Thio-UTP, is a potent agonist for P2Y2 and P2Y4 receptors. It is used in the study of crosslinking experiments, labeling of transcriptional complex. It also affects elongation and termination activity, causing transcriptional pause.