| 包装 | 价格(元) |
| 10ul (100 mM) | 电议 |
| 50ul (100 mM) | 电议 |
| 100ul (100 mM) | 电议 |
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 481.1 (free acid) |
| Formula | C10H18N3O13P3(free acid) |
| Synonyms | 5-Methyl-2'-deoxycytidine-5'-Triphosphate, m5-dCTP, 5-m-dCTP, 5m-dCTP |
| Chemical Name | lithium (5-(4-amino-5-methyl-2-oxopyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl triphosphate |
| Canonical SMILES | NC(C(C)=CN1C2CC(O)C(COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O2)=NC1=O.[Li+].[Li+].[Li+].[Li+] |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
5-Methyl-dCTP is a base modified deoxynucleotide. Methylation of the C-5 position of cytosines is a common and important epigenetic modification, with more than 4% of the cytosines present in the human genome reported to be methylated. This modification can be incorporated into DNA via the polymerase chain reaction (PCR) with 5-methyl-dCTP instead of dCTP. The ability to synthesize a defined, fully 5-methyl-dCTP-substituted DNA by PCR will be a valuable resource for a variety of applications including testing restriction endonucleases for methyl sensitivity and determining the specificity of 5-methyl-dCTP-dependent endonucleases. In addition, electroporation of 5-methyl-dCTP into living cells can also be incorporated into genomic DNA, and heritably alter cytosine methylation patterns and gene expression.
References:
1. Kumar S, Chinnusamy V, Mohapatra T. Epigenetics of Modified DNA Bases: 5-Methylcytosine and Beyond. Frontiers in Genetics, 2018, 9: 640.
2. Wong KK, McClelland M. PCR with 5-methyl-dCTP replacing dCTP. Nucleic Acids Research, 1991, 19(5): 1081-1085.
3. Lao VV, Herring JL, Kim CH, Darwanto A, Soto U, Sowers LC. Incorporation of 5-chlorocytosine into mammalian DNA results in heritable gene silencing and altered cytosine methylation patterns. Carcinogenesis, 2009, 30(5): 886-893.
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