Concanavalin A

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Concanavalin A

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

11028-71-0

包装与价格：

包装	价格(元)
50mg	电议
100mg	电议
250mg	电议

产品介绍

化学性质

Physical Appearance	A lyophilized powder
Storage	Store at -20°C
Cas No.	11028-71-0
Solubility	insoluble in DMSO; insoluble in EtOH; ≥51.5 mg/mL in H2O
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Concanavalin A a mannose/glucose-binding lectin originally extracted from Jack beans (Canavalia ensiformis) [1].

Concanavalin A is a well-known T cell mitogen that can activate the immune system, recruit lymphocytes and elicit cytokine production [1]. In addition to its mitogenic activity, concanavalin A also can induce programmed cell death via mitochondria-mediated apoptosis and autophagy [2].

In human diploid fibroblasts, concanavalin A treatment at 50 μg/ml for 0.5, 1, 3, 5, and 24 h, increased the proportion of TUNEL⁺ ve cells in a time-dependent manner. After 3 h, concanavalin A reduced mitochondrial membrane potential in a subpopulation of apoptotic cells with smaller cell volume and with apoptotic nuclear morphology. The measurement of intracellular calcium ion concentration ([Ca²⁺]_i) showed that concanavalin A did not affect [Ca²⁺]_i viable cells but induced a progressive depletion of [Ca²⁺]_i with generation of calcium oscillations in apoptotic cells [2].

Concanavalin A (i.v., twice at 3-day intervals) at a dose of 20 mg/kg inhibited liver tumor formation in NOD/LtSz-PrkdcJ mice at 21 days after tumor inoculation, whereas the doses of 10 and 15 mg/kg showed no effect.

References:

[1]. Dwyer J M, Johnson C. The use of concanavalin A to study the immunoregulation of human T cells. Clinical and Experimental Immunology, 1981, 46(2): 237-249.

[2]. Kulkarni G V, Lee W, Seth A, et al. Role of mitochondrial membrane potential in concanavalin A-induced apoptosis in human fibroblasts. Experimental Cell Research, 1998, 245(1): 170-178.

[3]. Chang C P, Yang M C, Liu H S, et al. Concanavalin A induces autophagy in hepatoma cells and has a therapeutic effect in a murine in situ hepatoma model. Hepatology, 2007, 45(2): 286-296.