资料参考

Human B cell culture and expansion kit is for activation and expansion of human B cells. Human monoclonal antibodies isolated for specific antibodies are widely used in immunity studies, historical studies and therapeutic development. Memory B cells derived from peripheral blood will produce high concentration of IgG in the supernatant under the stimulation of CD40L with other growth factors [1]. The B Cell Expansion Kit contains special treated feeder cells, expressing CD40L in activated form as well as human cell factors A and B potently inducing antibody-secret. The monoclonal antibody screening can be completed within 2 weeks with the help of the kit.

Reference:

[1] J. Huang, N. A. Doria-Rose, N. S. Longo, L. Laub, C.-L. Lin, E. Turk, B. H. Kang, S. A. Migueles, R. T. Bailer, J. R. Mascola, M. Connors, Isolation of Human Monoclonal Antibodies from Peripheral Blood B Cells. Nat. Protoc. 8, 1907-1915 (2013).