| CAS NO: | 210345-00-9 |
| 包装 | 价格(元) |
| 1mg | 电议 |
| 5mg | 电议 |
| Storage | Store at -20°C |
| M.Wt | 763.77 |
| Cas No. | 210345-00-9 |
| Formula | C37H42FN7O10 |
| Synonyms | Z-Trp-Glu(OMe)-His-Asp(OMe)-FMK |
| Solubility | insoluble in H2O; ≥26.32 mg/mL in EtOH with ultrasonic; ≥46.33 mg/mL in DMSO |
| Chemical Name | methyl (4S)-5-[[(2S)-1-[[(3S)-5-fluoro-1-methoxy-1,4-dioxopentan-3-yl]amino]-3-(1H-imidazol-5-yl)-1-oxopropan-2-yl]amino]-4-[[(2S)-3-(1H-indol-3-yl)-2-(phenylmethoxycarbonylamino)propanoyl]amino]-5-oxopentanoate |
| Canonical SMILES | COC(=O)CCC(C(=O)NC(CC1=CN=CN1)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)C(CC2=CNC3=CC=CC=C32)NC(=O)OCC4=CC=CC=C4 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
用pan-caspase抑制剂IV和炎性caspases抑制剂Z-WEHD-FMK处理感染的细胞，几乎完全阻断了沙眼衣原体引起的golgin-84的裂解。通过使用Z-WEHD-FMK处理感染的细胞阻断golgin-84的裂解，导致高尔基碎片的缺失和一些感染性细菌2-log的减少。
用Z-WEHD-FMK处理感染细胞，有效地阻止了高尔基体裂解，用DMSO作为对照，然后用荧光神经酰胺标记细胞。共聚焦图像显示，在DMSO处理的细胞中，神经酰胺迅速进入包涵体膜，并且在细菌膜内包涵体中积累。相反，Z-WEHD-FMK处理的细胞只有轻微的荧光，因为大多数脂质在包涵体外的高尔基体样结构中积累[1]。
通常的caspase抑制剂（Z-Asp-CHz-DCB）和capase-5抑制剂（Z-WEHD-FMK）不能诱导ECyd处理的rRNA裂解。Caspase-5（ICErei Ill/TY），是ICE蛋白酶的成员，激活的通路可能与ECyd诱导rRNA裂解有关[2]。
参考文献：
[1]. D. Heuer, A.R. Lipinskiet al.Chlamydia causes fragmentation of the Golgi compartment to ensure reproduction.NATURE, 457, 2009
[2]. Kamada, S., Funahashi, Y. and Tsujimoto, Y. (1997)Cell Death Diff. 4, 473-478.
| Cell experiment:[1] | |
Cell lines | Chlamydia trachomatis(C. trachomatis)-infected HeLa cells |
Reaction Conditions | 80 μM Z-WEHD-FMK for 9 h incubation |
Applications | Z-WEHD-FMK (80 μM; 9 h) elicited a near-complete blockage of C.trachomatis-induced cleavage of golgin-84 and increased GM130 expression in cells. Z-WEHD-FMK treatment resulted in a 2-log reduction in numbers of infectious bacteria, which could be reversed by knocking down golgin-84. Thus, Z-WEHD-FMK could effectively prevent Golgi fragmentation, and was used to explore the role of fragmentation of the Golgi compartment inChlamydiareproduction. |
Note | The technical data provided above is for reference only. |
References: 1. Heuer D, Rejman Lipinski A, Machuy N, et al. Chlamydia causes fragmentation of the Golgi compartment to ensure reproduction. Nature, 2009, 457(7230): 731-735. | |
| 一种合成肽，不可逆地抑制caspase-5及相关caspase的活性。 | ||||||
| 靶点 | caspase-5 | |||||
| IC50 | ||||||
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