Solasodine

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Solasodine

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

126-17-0

包装与价格：

包装	价格(元)
100mg	电议
250mg	电议

产品介绍

化学性质

Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	413.64
Cas No.	126-17-0
Formula	C₂₇H₄₃NO₂
Synonyms	Purapuridine;Solancarpidine;Solasodin
Solubility	insoluble in DMSO; insoluble in H2O; ≥20.7 mg/mL in EtOH
Chemical Name	5',6a,8a,9-tetramethyl-1,3,4,5,6,6a,6b,7,8,8a,8b,9,11a,12,12a,12b-hexadecahydrospiro[naphtho[2',1':4,5]indeno[2,1-b]furan-10,2'-piperidin]-4-ol
Canonical SMILES	[H]C(C1([H])C(C(C([H])([H])C([H])([H])C2([H])O[H])(C([H])([H])[H])C(C2([H])[H])=C([H])C1([H])[H])([H])C([H])([H])C3([H])[H])(C4([H])[H])C3(C([H])([H])[H])C(C5([H])C([H])([H])[H])([H])C4([H])OC65N([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])C6([H])[H]
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Description: IC50 Value: 12.17 ± 3.3 uM (Hela cell line)[1] Solasodine is a poisonous alkaloid chemical compound that occurs in plants of the Solanaceae family. Solasodine showed selective cytotoxicity against cervical cancer cell line (HeLa) and human myeloid leukemia cell line (U937). in vitro: Mouse embryonic teratocarcinoma P19 cells exposed to solasodine for 2 days followed by a 5-day washout differentiated into cholinergic neurons that expressed specific neuronal markers and displayed important axonal formation that continued growing even 30 days after treatment [2]. in vivo: A 2-week infusion ofsolasodine into the left ventricle of the rat brain followed by a 3-week washout resulted in a significant increase in bromodeoxyuridine uptake by cells of the ependymal layer, subventricular zone, and cortex that co-localized with doublecortin immunostaining, demonstrating the proliferative and differentiating properties of solasodine on neuronal progenitors. Solasodine treatment in rats resulted in a dramatic increase in expression of the cholesterol- and drug-binding translocator protein in ependymal cells, suggesting a possible role played by neurosteroid production in solasodine-induced neurogenesis. In GAD65-GFP mice that express the green fluorescent protein under the control of the glutamic acid decarboxylase 65-kDa promoter, solasodine treatment increased the number of GABAergic progenitors and neuroblasts generated in the subventricular zone and present in the olfactory migratory tract [2]. intraperitoneal (i.p.) injection of solasodine (25 mg/kg) significantly delayed (p < 0.01) latency of hind limb tonic extensor (HLTE) phase in the PCT-induced convulsions. In the MES model, solasodine significantly reduced (p < 0.001) duration of HLTE at 25, 50, and 100 mg/kg, i.p. in a dose-dependent manner [3]. Oral administration (80 mg/kg body wt/day for 30 days) of solasodine (extracted and isolated from the berries of the Solanum xanthocarpum) to intact dogs significantly decreased the epithelial cell height of cauda epididymides [4]. Toxicity: N/A Clinical trial: N/A

试验操作

Cell experiment:[1]
Cell lines	HCT116 cells
Reaction Conditions	20 and 40 μmol/L solasodine for 48 h incubation
Applications	Solasodine significantly inhibited the potential of colony spheroid formation strengthened by TGF-β1 in a concentration-dependent manner. Meanwhile, the mRNA and protein levels of stemness markers including CD133, CD44, Nanog, Oct-4 and Sox-2 were down-regulated with increasing concentrations of solasodine.
Animal experiment:[1]
Animal models	Solasodine significantly inhibited the potential of colony spheroid formation strengthened by TGF-β1 in a concentration-dependent manner. Meanwhile, the mRNA and protein levels of stemness markers including CD133, CD44, Nanog, Oct-4 and Sox-2 were down-regulated with increasing concentrations of solasodine.
Dosage form	30 and 50 mg/kg Once daily by intraperitoneal injection for 5 weeks
Applications	Tumor xenografts in both low- (30 mg/kg) and high-dose (50 mg/kg) solasodine groups grew more slowly, and the average volume and weight of the finally resected tumors in solasodine-treated groups were remarkably lower. In harvested tumors, stemness and epithelial-mesenchymal transition (EMT) related molecules were both down-regulated. The results of this study implied that solasodine may be a novel therapeutic drug for human colorectal cancer treatment.
Note	The technical data provided above is for reference only.
	References: 1. Zhuang YW, Wu CE, Zhou JY, et al. Solasodine reverses stemness and epithelial-mesenchymal transition in human colorectal cancer. Biochemical and Biophysical Research Communications, 2018, 505(2): 485-491.