| 包装 | 价格(元) |
| 5mg | 电议 |
| 10mg | 电议 |
| 50mg | 电议 |
Cell lines | Human fibroblast |
Preparation Method | Aspire the cell culture media from the cells and add 1 ml of FITC-Dextran containing medium and incubate for 3 days at 37 ℃ with 5% of CO2 in air. |
Reaction Conditions | 1mL 0.1 mg/ml FITC-Dextran for 3 days at 37 ℃ |
Applications | When analyse the lysosomal pH in cultured cells using the fluorescent probe fluorescein isothiocyanate (FITC)-dextran together with a dual-emission ratiometric technique suitable for flow cytometry. Fluorescence-labeled dextran is endocytosed and accumulated in the lysosomal compartment. FITC shows a pH-dependent variation in fluorescence when analyzed at maximum emission wavelength and no variation when analyzing at the isosbestic point, thereby the ratio can be used to determine the lysosomal pH. |
Animal models | Specific-pathogen-free male C3H/HeOuJ mice |
Preparation Method | The 12-week-old male mice were fasted for 6 h and 4-kDa FITC-Dextran or 70-kDa FITC-Dextran was applied orally at a single dose. Three mice each were anaesthetized after 30 min, 1, 1.5, 2 and 8 h; another three mice were anaesthetized after 15 and 45 min and blood from the retrobulbar capillary plexus was sampled into heparinized tubes for 4-kDa FITC-Dextran analyses. Plasma was obtained after centrifugation at 2000 g for 5 min. |
Dosage form | 600 mg/kg 4-kDa FITC dextran;(100 μL, 5 mg/mL PBS)70-kDa FITC-Dextran for 30min-8h |
Applications | After 6 h of fasting in our C3H/HeOu mice, plasma concentrations of 4-kDa FITC-Dextran peaked at 45 min after oral administration. |
| 产品描述 | FITC-Dextran is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). In cell permeability studies, FITC-Dextran can assess BBB permeability and BBB disruption[1,2]. When analyse the lysosomal pH in cultured cells using the fluorescent probe fluorescein isothiocyanate (FITC)-dextran together with a dual-emission ratiometric technique suitable for flow cytometry. Fluorescence-labeled dextran is endocytosed and accumulated in the lysosomal compartment. FITC shows a pH-dependent variation in fluorescence when analyzed at maximum emission wavelength and no variation when analyzing at the isosbestic point, thereby the ratio can be used to determine the lysosomal pH[2].Fitc-glucan increased intestinal epithelial paracellular permeability after 21 days of incubation[5]. When examined their expression and FITC-Dextran uptake by various human DC preparations. In contrast to immature Mo-DC, the FITC-dextran uptake by LC was not inhibited effectively by mannose, an inhibitor for MMR-mediated FITC-dextran uptake[7]. After 6 h of fasting in our C3H/HeOu mice, plasma concentrations of 4-kDa FITC-Dextran peaked at 45 min after oral administration[3]. The fluorescence of small vessels and neovascular tufts could be observed clearly following RO injection of 0.05 ml of 25 mg/ml or 50 mg/ml FITC-dextran. No visible damage to tissues adjacent to the injection site was discovered. Vitreous blood flow was gradually reduced from P0 to P5 and eventually disappeared in P17 OIR mice, as demonstrated by FITC-dextran perfusion. The retinal NV areas assessed by isolectin B4 were larger than those assessed by FITC-dextran, but the retinal avascular areas were smaller[4]. When measured fluorescence recovery after photobleaching (FRAP) of fluorescein isothiocyanate (FITC)-labelled 10 and 250 kDa dextran (FITC-Dextran) in isolated rat descending colonic crypts.FRAP of either 10 or 250 kDa FITC-Dextran in crypt lumens was almost complete within 2-3 min. In the presence of amiloride (0.1 mM), or in the absence of Na+, the rate of FITC-Dextran uptake into the crypt lumens was reduced by 70-80 %[6]. References: |
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