| CAS NO: | 1239-45-8 |
| 包装 | 价格(元) |
| 10mM (in 1mL DMSO) | 电议 |
| 250mg | 电议 |
Ethidiumbromide常用作分子生物实验学中的荧光标记(核酸染色)，如琼脂糖凝胶电泳。
| Cas No. | 1239-45-8 |
| 别名 | 溴化乙啶,EtBr; Homidium bromide |
| Canonical SMILES | CC[N+]1=C(C2=CC=CC=C2)C3=CC(N)=CC=C3C4=C1C=C(N)C=C4.[Br-] |
| 分子式 | C21H20BrN3 |
| 分子量 | 394.31 |
| 溶解度 | Water: ≥ 100 mg/mL (253.61 mM); DMSO: 83.33 mg/mL (211.33 mM) |
| 储存条件 | Store at RT,protect from light |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. |
| Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
| 产品描述 | Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).The preparation of agarose gel:1. Agarose gels are commonly used in concentrations of 0.5% to 2.5% depending on the size of bands needed to be separated.2. Mix the agarose powder with 1X TAE/TBE.3. Microwave for 1-3 min until the agarose is completely dissolved (Caution: not overboil).4. Make the solution cool down before solidification.5. Add ethidium bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (Stocks are generally 10 mg/ml, and require 5 µL stock/100 mL gel).6. Ethidium bromide binds to the DNA and you could visualize the DNA under ultraviolet (UV) light.CAUTION: EtBr is a known mutagen. Please pay attention to strengthening protection. [1]. P Stevenson, et al. Comparison of isometamidium chloride and homidium bromide as prophylactic drugs for trypanosomiasis in cattle at Nguruman, Kenya. Acta Trop. 1995 May;59(2):77-84. [2]. Sabnis, Ram Wasudeo (2010). Handbook of Biological Dyes and Stains: Synthesis and Industrial Application. Hoboken, NJ: Wiley. ISBN 978-0-470-40753-0. |
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