3-Nitropropionic acid

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CAS NO:

504-88-1

包装与价格：

包装	价格(元)
10mM (in 1mL DMSO)	电议
50mg	电议

产品介绍

化学性质

Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	119.08
Cas No.	504-88-1
Formula	C₃H₅NO₄
Solubility	≥103 mg/mL in H2O; ≥114.2 mg/mL in EtOH; ≥6 mg/mL in DMSO
Chemical Name	3-nitropropanoic acid
Canonical SMILES	OC(CC[N+]([O-])=O)=O
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

3-Nitropropionic acid is an irreversible inhibitor of mitochondrial respiratory complex II (succinate dehydrogenase), which impairs cellular energy metabolism by inhibiting succinate dehydrogenase and reducing ATP production. Also, 3-nitropropionic acid can cause the generation and release of reactive oxygen species (ROS) from mitochondria (the main source of cellular oxidative stress), mitochondrial DNA damage, as well as loss of mitochondrial function.

References:

1. Huang LS, Sun G, Cobessi D, et al. 3-Nitropropionic acid is a suicide inhibitor of mitochondrial respiration that, upon oxidation by complex II, forms a covalent adduct with a catalytic base arginine in the active site of the enzyme. Journal of Biological Chemistry, 2006, 281(9): 5965-5972.

2. Zhang JQ, Shen M, Zhu CC, et al. 3-Nitropropionic acid induces ovarian oxidative stress and impairs follicle in mouse. PLoS One, 2014, 9(2): e86589.

3. Mandavilli BS, Boldogh I, Van Houten B. 3-Nitropropionic acid-induced hydrogen peroxide, mitochondrial DNA damage, and cell death are attenuated by Bcl-2 overexpression in PC12 cells. Brain research. Molecular brain research, 2005, 133(2): 215-223.

试验操作

Cell experiment:[3]
Cell lines	PC12 cells
Reaction Conditions	4 or 8 mM 3-nitropropionic acid for 48 h incubation
Applications	3-Nitropropionic acid induced cytotoxicity by triggering ROS production and mitochondrial DNA damage, which could be avoided by overexpressing Bcl-2 in PC12 cells.
Animal experiment:[2]
Animal models	Female ICR mice
Dosage form	6.25, 12.5 and 25 mg/kg Continuous intraperitoneal injection for 7 days
Applications	3-Nitropropionic acid induced granulosa cell apoptosis, large follicle atresia, and an increase of ROS levels in the ovary. Thus, 3-nitropropionic acid could be used to establish an in vivo model of ovarian oxidative stress for studying the mechanism of resulting damage induced by free radicals and for the screening of novel antioxidants.
Note	The technical data provided above is for reference only.
	References: 1. Huang LS, Sun G, Cobessi D, et al. 3-Nitropropionic acid is a suicide inhibitor of mitochondrial respiration that, upon oxidation by complex II, forms a covalent adduct with a catalytic base arginine in the active site of the enzyme. Journal of Biological Chemistry, 2006, 281(9): 5965-5972. 2. Zhang JQ, Shen M, Zhu CC, et al. 3-Nitropropionic acid induces ovarian oxidative stress and impairs follicle in mouse. PLoS One, 2014, 9(2): e86589. 3. Mandavilli BS, Boldogh I, Van Houten B. 3-Nitropropionic acid-induced hydrogen peroxide, mitochondrial DNA damage, and cell death are attenuated by Bcl-2 overexpression in PC12 cells. Brain research. Molecular brain research, 2005, 133(2): 215-223.