Wiskostatin

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Wiskostatin

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

253449-04-6

包装与价格：

包装	价格(元)
1mg	电议
5mg	电议
10mg	电议
50mg	电议

产品介绍

化学性质

Physical Appearance	A crystalline solid
Storage	Store at -20°C
M.Wt	426.15
Cas No.	253449-04-6
Formula	C₁₇H₁₈Br₂N₂O
Solubility	Soluble in DMSO
Chemical Name	(R)-1-(3,6-dibromo-9H-carbazol-9-yl)-3-(dimethylamino)propan-2-ol
Canonical SMILES	BrC1=CC=C(C2=C1)N(C(C2=C3)=CC=C3Br)C[C@@H](CN(C)C)O
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Wiskostatin is an N-WASP inhibitor, with an IC50 value slightly less than 10 μM. Wiskostatin interacts with a cleft in the regulatory GTPase-binding domain (GBD) of N-WASP and maintains N-WASP in an inactive conformation, thereby inhibiting Cdc42 and phosphatidylinositol 4,5-bisphosphate (PIP₂) mediated N-WASP activation, as well as Arp2/3 complex activation. N-WASP, highly expressed in the brain, belongs to the WASP family in which member proteins initiate the nucleation of new actin filaments through activation of the Arp2/3 complex, and thus are emerging as critical regulators of the actin cytoskeleton. By inhibiting N-WASP, wiskostatin has been shown to interfere with the formation of dendritic spines and synapses in neurons.

References:

1. Peterson JR, Bickford LC, Morgan D, et al. Chemical inhibition of N-WASP by stabilization of a native autoinhibited conformation. Nature Structural & Molecular Biology, 2004, 11(8): 747-755.

2. Wegner AM, Nebhan CA, Hu L, et al. N-wasp and the arp2/3 complex are critical regulators of actin in the development of dendritic spines and synapses. Journal of Biological Chemistry, 2008, 283(23): 15912-15920.

试验操作

Cell experiment:[2]
Cell lines	Hippocampal neurons
Reaction Conditions	2 or 5 μM wiskostatin
Applications	Wiskostatin caused a dose-dependent decrease in the number of dendritic spines and excitatory synapses. However, wiskostatin, even at the highest concentration (5 μM), did not significantly affect the number of inhibitory synapses. In addition, wiskostatin did not adversely affect the health of the neurons, because they were observed to develop normally and no detectable effects on the growth of axons or dendrites were seen at any of the wiskostatin concentrations used.
Note	The technical data provided above is for reference only.
	References: 1. Peterson JR, Bickford LC, Morgan D, et al. Chemical inhibition of N-WASP by stabilization of a native autoinhibited conformation. Nature Structural & Molecular Biology, 2004, 11(8): 747-755. 2. Wegner AM, Nebhan CA, Hu L, et al. N-wasp and the arp2/3 complex are critical regulators of actin in the development of dendritic spines and synapses. Journal of Biological Chemistry, 2008, 283(23): 15912-15920.