| 包装 | 价格(元) |
| 10ul (100 mM) | 电议 |
| 50ul (100 mM) | 电议 |
| 100ul (100 mM) | 电议 |
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 497.1 (free acid) |
| Formula | C10H18N3O14P3(free acid) |
| Synonyms | 5-Methylcytidine-5'-Triphosphate, 5-Me-CTP, 5mCTP, 5-mCTP |
| Canonical SMILES | [NH2]=C(C(C)=CN1C2C(O)C(O)C(COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O2)=NC1=O.[Li+].[Li+].[Li+].[Li+] |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
5-Methyl-CTP is a modified nucleoside triphosphate, commonly used to substitute the naturally occurring cytidine triphosphate (CTP) duringin vitromRNA synthesis. Methylation of the cytosine C-5 position is a common, post-transcriptional modification for mRNA. Incorporation of chemically modified 5-methyl-CTP can increase mRNA stability and protein expression, and meanwhile, reduce interaction of innate immune receptors within vitro-transcribed (IVT) mRNA. Apart from its application in encoding antigens for the activation of immune response against a variety of diseases such as COVID-19, IVT mRNA can also express the nanobody/variable domain of heavy chain of heavy-chain antibody targeting the intracellular components, enabling intracellular antigen labeling and manipulation.
References:
1. Rahman MM, Zhou N, Huang J. An overview on the development of mRNA-based vaccines and their formulation strategies for improved antigen expression in vivo. Vaccines (Basel), 2021, 9(3): 244.
2. Lu J, Lu G, Tan S, et al. A COVID-19 mRNA vaccine encoding SARS-CoV-2 virus-like particles induces a strong antiviral-like immune response in mice. Cell Research, 2020, 30(10): 936-939.
3. Zhou X, Hao R, Chen C, et al. Rapid delivery of nanobodies/VHHsinto living cells via expressing in vitro-transcribed mRNA. Molecular Therapy - Methods & Clinical Development, 2020, 17: 401-408.
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