| CAS NO: | 1221498-88-9 |
| 包装 | 价格(元) |
| 10ul (5 mM) | 电议 |
| 200ul (5 mM) | 电议 |
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 887.44 |
| Cas No. | 1221498-88-9 |
| Formula | C28H44Li4N7O16P3S |
| Solubility | ≥100.7 mg/mL in H2O; ≥46.3 mg/mL in EtOH; ≥95.6 mg/mL in DMSO |
| Chemical Name | lithium (5-(4-amino-2-oxo-5-((E)-3-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)prop-1-en-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl triphosphate |
| Canonical SMILES | O=C1N[C@]2([H])[C@]([C@H](CCCCC(NCCCCCC(NC/C=C/C3=CN(C(NC3N)=O)C4OC(COP(OP(OP([O-])([O-])=O)([O-])=O)([O-])=O)C(C4)O)=O)=O)SC2)([H])N1.[Li+].[Li+].[Li+].[Li+] |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
Biotin-11-dUTP, a biotinylated deoxynucleotide triphosphate analogue, can be enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart deoxythymidine triphosphate (dTTP). The resulting biotin-labeled DNA/cDNA probes are then detected with streptavidin-horseradish peroxidase (HRP) conjugates, alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Biotin-11-dUTP is commonly used for PCR, nick translation, as well as reverse transcription. It is also suitable for apoptosis assays.
References:
1. Choi JH, Kim SY, Kim SK, et al. An Integrated Approach for Analysis of the DNA Damage Response in Mammalian Cells. Journal of Biological Chemistry, 2015, 290(48): 28812-28821.
2. Day PJ, Bevan IS, Gurney SJ, et al. Synthesis in vitro and application of biotinylated DNA probes for human papilloma virus type 16 by utilizing the polymerase chain reaction. Biochemical Journal, 1990, 267(1): 119-123.
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