| CAS NO: | 61135-33-9 |
| 包装 | 价格(元) |
| 100mg | 电议 |
| 1g | 电议 |
| Physical Appearance | Solution |
| Storage | Store at -20°C |
| M.Wt | 252.22 |
| Cas No. | 61135-33-9 |
| Formula | C11H12N2O5 |
| Solubility | ≥25.2 mg/mL in DMSO; insoluble in EtOH; ≥3.16 mg/mL in H2O with gentle warming and ultrasonic |
| Chemical Name | 5-ethynyl-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione |
| Canonical SMILES | O=C1C(C#C)=CN([C@@H]2O[C@H](CO)[C@@H](O)C2)C(N1)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
5-Ethynyl-2'-deoxyuridine (5-EdU) is a thymidine analog that can be incorporated into replicating DNA for detection of cell proliferation. 5-EdU can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle through click chemistry. Click chemistry is a method of covalently coupling an azide with an alkyne. 5-EdU has an alkyne handle that can be detected using azide-containing fluorescent probes through a highly efficient click chemistry reaction [1][2]. In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. 5-EdU-labelled cells may be isolated to determine the transcript of cells, from neural cancer or tissues that have undergone division in vitro and in vivo[3].
References：
[1] Zeng C, Pan F, et al. Evaluation of 5-ethynyl-2'-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system. Brain Res. 2010 Mar 10;1319:21-32.
[2] Cavanagh BL, Walker T, et al. Thymidine analogues for tracking DNA synthesis. Molecules. 2011 Sep 15;16(9):7980-93.
[3] Endaya BB, Lam PY, et al. Transcriptional profiling of dividing tumor cells detects intratumor heterogeneity linked to cell proliferation in a brain tumor model. Mol Oncol. 2016 Jan;10(1):126-37.
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