| 包装 | 价格(元) |
| 10mM (in 1mL DMSO) | 电议 |
| 25mg | 电议 |
Digitonin is a steroidal saponin used extensively as a mild non-ionic detergent.
Cell lines | Caco-2 and MCF-7 cells |
Preparation Method | The cells were incubated with 100 µl of medium containing different concentrations of digitonin and other secondary metabolites for 24 hours. |
Reaction Conditions | 0-500µM for 24 hours. |
Applications | Digitonin showed the high exhibition to Caco-2, MCF-7 with IC50 of 15.72 ± 0.16 and 5.23 ± 0.61µM, respectively. |
Animal models | Inbred rats of both sexes of the Lister-Hooded (LH) strain |
Preparation Method | Digitonin was dissolved in dimethylsulfoxide (DMSO) and diluted with saline to yield a solution that contained 400 µM digitonin and 40 µM DMSO; 0.2 ml of the solution was infused into the hepatic artery. |
Dosage form | 400 µM 0.2 ml of Digitonin solution, a catheter (PE 10, inner diameter 0.28 mm, outer diameter 0.61 mm) was inserted into the hepatic artery proper via the gastroduodenal artery |
Applications | After pretreatment with digitonin the platinum content in the tumour as measured at 1 h after CBDCA infusion was 66±19 ng/mg. This was more than 5 times higher than the values obtained after pretreatment with saline alone (12±4 ng/mg) or with DMSO-saline (10±2 ng/mg) |
| 产品描述 | Digitonin is a steroidal saponin used extensively as a mild non-ionic detergent[1]. Digitonin finds application as a cholesterol precipitating agent[2]. Digitonin increases cell permeability by binding specifically to cholesterol in the cell membrane[4]. Digitonin had high exhibition against Caco-2, MCF-7 with IC50 of 15.72 ± 0.16 and 5.23 ± 0.61μM, respectively. Combinations of the panel of SM with a non-toxic concentration of digitonin (2 or 5 μM) generally enhanced the cytotoxicity in Caco-2, MCF-7 cells[3]. Pretreatment with digitonin increases the tumour uptake of carboplatin and potentiates the cytotoxic effect of carboplatin[4]. References: |
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