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Di-8-ANEPPS
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Di-8-ANEPPS图片
包装与价格:
包装价格(元)
1mg电议
5mg电议

产品介绍
Di-8-ANEPPS是萘基苯乙烯基电压敏感染料,它们的荧光激发和发射光谱会因电压的变化而变化。

Cell experiment:

To implement the technique, cells enzymatically isolated from mouse flexor digitorum brevis (FDB) muscle are stained by brief exposure to a saline with Di-8-ANEPPS and then washed in solution[1].

产品描述

Di-8-ANEPPS is a naphthylstyryl voltage-sensitive dye, shifting both their fluorescence excitation and emission spectra upon changes in Vm .

A mouse cell staining with Di-8-ANEPPS is applied voltage clamp pulses and immersed in Na+-containing solution. The Di-8-ANEPPS signal, which largely reflects t system voltage, has an asymmetrical positive component upon application of depolarizing pulses. This is interpreted as reflecting a propagating action potential, escaping from the voltage-clamped plasma membrane. Its temporal properties are not unlike those of the "center" signal. Notably, the peak of the signal occurrs 0.3 ms after the leading edge of the depolarizing pulse[1].

[1]. Manno C, et al. Confocal imaging of transmembrane voltage by SEER of di-8-ANEPPS. J Gen Physiol. 2013 Mar;141(3):371-87.