描述
| Gene ID: | N/A | Accession #: | N/A |
| Alternate Names: | Proteina/g, protein ag, protein a/g, proteinag, protein-ag, protein-a/g, proteinag, Protein A/G, rProtein A/G, r-protein A/G, recombinant protein A/G, IgG purification, antibody purification, Protein A, Protein G, Protein A&G. |
| Source: | Escherichia Coli. | M.Wt: | 59.7 kDa |
| Appearance: | Lyophilized protein | Storage: | Store at -20°C |
| Formulation: | Lyophilized powder. |
| Solubility: | Reconstitution in water to a concentration of 5 mg/ml gives a clear solution.. |
| Shipping Condition: | Gel pack. | Handling: | Centrifuge the vial prior to opening. |
| Usage: | For Research Use Only! Not to be used in humans. |
资料参考
Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins. Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria. Protein A/G is a genetically engineered protein that combines the IgG binding profiles of both Protein A and Protein G. It is a gene fusion product. Recombinant fusion protein A/G contains 6× His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the fusion protein A/G to ensure the maximum specific IgG binding. 6× His-tag on N-terminus can be used for affinity purification or for protein A/G detection using anti-His-tag antibody.
Protein A/G binds to all IgG subclasses from various mammalian species, including all IgGs that bind to both Protein A and Protein G, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies.
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