| CAS NO: | 122266-55-1 |
| 包装 | 价格(元) |
| 100mg | 电议 |
| 1g | 电议 |
| Storage | Store at -20°C该产品在溶液中不稳定，建议现配现用 |
| M.Wt | 504.64 |
| Cas No. | 122266-55-1 |
| Formula | C19H28N4O6S3 |
| Synonyms | NHS-SS-Biotin,Biotin disulfide N-hydroxysuccinimide ester |
| Solubility | ≥28.79 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH |
| Chemical Name | 2,5-dioxopyrrolidin-1-yl 3-((2-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)ethyl)disulfanyl)propanoate |
| Canonical SMILES | O=C1CCC(N1OC(CCSSCCNC(CCCC[C@@H]2SC[C@@](N3)([H])[C@]2([H])NC3=O)=O)=O)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
NHS-SS-biotin [Succinimidyl-2-(biotinamido)-ethyl-1,3-dithiopropionate]是一个长链裂解的胺反应性生物素化试剂，由于结构中横桥的存在，它具有24.3 A长度的间隔臂。长间隔臂增加了共价修饰分子与双环生物素环之间的长度，从而增强分子与亲和素或链亲和素探针的结合能力。NHS-SS-biotin的NHS酯基与蛋白和其它分子的胺基反应形成酰胺键，并释放sulfo-NHS。NHS-SS-Biotin是水不溶性的分子，在加入反应性水溶液之前需要先溶于有机溶剂中。
参考文献：
Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
o Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
o Intracellular labeling—membrane-permeable, can be used to label inside cells
o Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the N-terminal-amine
o Cleavable—disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT
o Solubility—water insoluble, must be dissolved in DMSO or DMF before further dilution in aqueous buffers
o Medium length—spacer arm is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain
| Biotinylation method [1]: | |
Sample | hippocampal neurons |
Preparation method | Soluble in DMSO or DMF. |
Reaction Conditions | 1.5 mg/ml, 4 ℃ for 1 h |
Applications | Neurons were washed with the artificial cerebrospinal fluid（ACSF） at 37 ℃, and incubated with 1.5 ml of 1.5 mg/ml NHS-SS-biotin with gentle shaking at 4 ℃ for 1 h. After washing, neurons were switched to the neuronal culture medium and incubated at 37 ℃. At indicated times of incubation, neurons were cooled to 4 ℃ and un-endocytosed surface biotin was cleaved by incubating in the glutathione cleavage buffer (50 mM glutathione, 75 mM NaCl, 10 mM EDTA, 1% BSA, and 0.075 N NaOH). Neurons were lysed in the modified RIPA buffer (50 mM Tris–HCl, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxylate, 1 mM EDTA, and protease inhibitors). Lysates were cleared by centrifugation at 10,000g for 10 min at 4 ℃ and incubated at 4 ℃ over-night with 70 μl of 50% streptavidin beads. Endocytosis of ErbB proteins was assayed using cleavable biotin. Bead-associated proteins were subjected to Western blot analysis. |
References: [1]. Yu Liu, Yan-Mei Tao, Ran-Sook Woo, Wen-Cheng Xiong, Lin Mei. Stimulated ErbB4 internalization is necessary for neuregulin signaling in neurons. Biochemical and Biophysical Research Communications 354 (2007) 505–510. | |
| 描述 | NHS-SS-Biotin是一种细胞通透性的和伯胺反应性的生物素化试剂。 | |||||
| 靶点 | proteins | |||||
| IC50 | ||||||
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