| CAS NO: | 254109-22-3 |
| 包装 | 价格(元) |
| 1mg | 电议 |
| 5mg | 电议 |
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 496.42 |
| Cas No. | 254109-22-3 |
| Formula | C25H18F2N2O7 |
| Solubility | Soluble in DMSO |
| Chemical Name | 4-amino-2',7'-difluoro-5-(methylamino)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate |
| Canonical SMILES | O=C1OC2(C3=CC(F)=C(C=C3OC4=C2C=C(C(OC(C)=O)=C4)F)OC(C)=O)C5=C1C(N)=C(NC)C=C5 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
DAF-FM diacetate is a cell-permeable, fluorescent probe for the detection and bioimaging of nitric oxide (NO), with excitation/emission maxima of 495/515 nm. It passively diffuses across cellular membranes and, once inside cells, is then deacetylated by intracellular esterases to DAF-FM [1].
The fluorescence quantum yield of DAF-FM was ~ 0.005, but increased about 160 folds, to ~ 0.81, after reacting with NO [1]. DAF-FM fluorescence with NO is time-dependent. In a reaction mixture containing 190 nM NO or 380 nM NO, and 1 μM DAF-FM, relative fluorescence (493 nm excitation, 514 nm emission) increased rapidly during the first 10 min of incubation and reached a steady slow increase at 40 min. After 60 min, there was a minimal (~ 15%) increase in fluorescence [2].
DAF-FM diacetate exhibited several advantages over the NO probe, DAF-2 diacetate. When the pH values exceeded 5.5, the spectra of the NO adduct of DAF-FM become independent. Compared with the NO adduct of DAF-2, the combination of NO and DAF-FM was significantly more photostable. In addition, the NO detection limit of DAF-FM was ~ 3 nM, more sensitive than that of DAF-2 (~ 5 nM) [1, 3].
References:
[1]. Kojima H, Urano Y, Kikuchi K, et al. Fluorescent indicators for imaging nitric oxide production. Angewandte Chemistry International Edition English, 1999, 38(21): 3209-3212.
[2]. Vitecek J, Reinohl V, Jones R L. Measuring NO production by plant tissues and suspension cultured cells. Molecular Plant, 2008, 1(2): 270‐284.
[3]. Kojima H, Nakatsubo N, Kikuchi J, et al. Detection and imaging of nitric oxide with novel fluorescent indicators: Diaminofluoresceins. Analytical Chemistry, 1998, 70(13): 2446-2453.
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