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Cy3 azide
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Cy3 azide图片
包装与价格:
包装价格(元)
5mg电议
25mg电议

产品介绍

化学性质

Storage24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
M.Wt752.88
FormulaC34H45N6NaO8S2
Solubility≥10 mg/mL in DMSO; ≥16.67 mg/mL in EtOH; ≥16.67 mg/mL in H2O
Chemical Name(2E)-1-[6-(3-azidopropylamino)-6-oxohexyl]-3,3-dimethyl-2-[(E)-3-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)prop-2-enylidene]indole-5-sulfonate
Canonical SMILESCC1(C2=C(C=CC(=C2)S(=O)(=O)[O-])[N+](=C1C=CC=C3C(C4=C(N3CCCCCC(=O)NCCCN=[N+]=[N-])C=CC(=C4)S(=O)(=O)[O-])(C)C)C)C
运输条件蓝冰运输或根据您的需求运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。

资料参考

Sulfo-Cy3 azide is a sulfonated, hydrophilic and highly water soluble dye for Click Chemistry. For labeling of various molecules in aqueous phase for brightness and photostability of fluorophore, sulfo-Cy3 azide is an ideal choice. Click reaction can be carried out in pure water without need to use organic co-solvent. For labeling proteins and even intact biological objects, no organic co-solvent is needed. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye–dye interactions.

In vitro, sulfo-Cy3 azide was used for fluorescent microscopy staining. Human U87MG glioblastoma cells that overexpress uPAR were just stained by Cy3-AE105 conjugate [1].

Reference:
[1] Sun, L.  Gai, Y. Anderson, C.J.; Zeng, D. Highly-efficient and versatile fluorous-tagged Cu (I)-catalyzed azide–alkyne cycloaddition ligand for preparing bioconjugates. Chemical Communications, 2015, 51, 17072–17075.

Spectral properties

最大激发光波 (nm)563最大发射光波 (nm)584
消光系数 (M-1cm-1)162000量子产量0.1
CF2600.03CF2800.06