Cell lines

A murine pancreatic β cell line, INS-1

Reaction Conditions

15 or 30 mM streptozocin for 1 h incubation

Applications

Higher rates of apoptosis, as compared to necrosis, were observed when cells were exposed to 15 mM streptozocin for 1 h followed by a 24 h recovery period. Higher doses of streptozocin (30 mM) caused the cells to undergo necrosis (22%) as well as apoptosis (17%). Streptozotocin at low doses induced apoptosis and at high doses caused necrosis in INS-1 cells.

Animal models

Male adult Holtzman rats

Dosage form

50, 65 or 100 mg/kg

A single intravenous injection

Applications

Streptozotocin (65 mg/kg) resulted in rapid degranulation of β cells, accumulation of glycogen in the proximal convoluted tubules of the kidney, as well as development of cataracts in 4 months after streptozotocin treatment. At a higher dose of 100 mg/kg, streptozotocin produced lesions in the exocrine cells of the pancreas, and led to persistence of small, possibly secretory, granules in the Golgi zone of β cells in diabetic rats. Streptozotocin is often used to induce diabetes mellitus in experimental animals.

Note

The technical data provided above is for reference only.

References:

1. Saini KS, Thompson C, Winterford CM, et al. Streptozotocin at low doses induces apoptosis and at high doses causes necrosis in a murine pancreatic beta cell line, INS-1. Biochemistry and Molecular Biology International, 1996, 39(6): 1229-1236.

2. Arison RN, Ciaccio EI, Glitzer MS, et al. Light and electron microscopy of lesions in rats rendered diabetic with streptozotocin. Diabetes, 1967, 16(1): 51-56.