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Ionomycin calcium salt
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Ionomycin calcium salt图片
CAS NO:56092-82-1
包装与价格:
包装价格(元)
5mg电议
10mg电议

产品介绍

化学性质

Physical AppearanceA crystalline solid
StorageDesiccate at -20°C
M.Wt747.08
Cas No.56092-82-1
FormulaC41H70O9·Ca
SolubilitySoluble in DMSO
Chemical Namecalcium (4S,6S,8S,10E,12R,14R,16E,18S,19S,20R,21S)-19,21-dihydroxy-22-((2R,2'R,5R,5'S)-5'-((R)-1-hydroxyethyl)-2,5'-dimethyloctahydro-[2,2'-bifuran]-5-yl)-4,6,8,12,14,18,20-heptamethyl-11-oxido-9-oxodocosa-10,16-dienoate
Canonical SMILESO[C@H](C)[C@@]1(C)O[C@H](CC1)[C@]2(C)O[C@@H](C[C@@H]([C@@H](C)[C@H]([C@H](/C=C/C[C@@H](C)C[C@@H](C)/C([O-])=C\C([C@@H](C)C[C@@H](C)C[C@@H](C)CCC([O-])=O)=O)C)O)O)CC2.[Ca+2]
运输条件蓝冰运输或根据您的需求运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。

资料参考

钙离子(钙)信号通路在受精中发挥主要作用,精子不仅提供其遗传物质,也使细胞内钙离子浓度升高,这对于完成卵母细胞活化和胚胎发育是必要的。离子霉素是由链霉菌产生的离子载体。工业上,离子霉素通常为游离酸或钙盐。

体外实验:离子霉素是用于研究胞内钙水平的提高和研究跨膜钙转运的工具。它也可以用来刺激胞内产生细胞因子,如干扰素、穿孔素、IL-2和IL-4。这些细胞因子在炎症反应中发挥重要作用[1]。

在体实验:10 μmol/l的离子霉素有效激活卵母细胞的活化和随后的发育效率。二次给予5 μmol/l离子霉素明显改善囊胚发育。在体外或体内小鼠胚胎发育中使用离子霉素无副作用[1]。

临床试验:截至目前,离子霉素仍处于临床前开发阶段。

参考文献:
[1] Heytens E, Soleimani R, Lierman S, De Meester S, Gerris J, Dhont M, Van der Elst J, De Sutter P. ? Effect of ionomycin on oocyte activation and embryo development in mouse. Reprod Biomed Online. 2008 Dec;17(6):764-71.

试验操作

Cell experiment:[1]

Cell lines

Human bladder cancer cell line HT1376

Reaction Conditions

0 ~ 100 μg/ml ionomycin for 0 ~ 96 h incubation

Applications

Thein vitrogrowth rate of HT1376 cells was suppressed by ionomycin in a dose- and time-dependent manner. After continuous treatment with 10 μg/ml ionomycin, characteristic apoptotic DNA degradation, as well as marked decreases in the ratios of Bcl-2 to Bax mRNA and protein could be observed in HT1376 cells.

Animal experiment:[1]

Animal models

Athymic nude mice bearing HT1376 tumors

Dosage form

50 μg of ionomycin in 100 μl PBS

Injected intratumorally three times weekly for 4 weeks

Applications

Intratumoral injection of ionomycin into subcutaneous HT1376 tumors reduced the tumorigenicity in nude mice. Furthermore, thesein vivogrowth-inhibitory effects of ionomycin were significantly enhanced by pretreatment with cisplatin.

Note

The technical data provided above is for reference only.

References:

1. Miyake H, Hara I, Yamanaka K, et al. Calcium ionophore, ionomycin inhibits growth of human bladder cancer cells both in vitro and in vivo with alteration of Bcl-2 and Bax expression levels. The Journal of Urology, 1999, 162(3 Pt 1): 916-921.