CAS NO: | 56092-82-1 |
包装 | 价格(元) |
5mg | 电议 |
10mg | 电议 |
Physical Appearance | A crystalline solid |
Storage | Desiccate at -20°C |
M.Wt | 747.08 |
Cas No. | 56092-82-1 |
Formula | C41H70O9·Ca |
Solubility | Soluble in DMSO |
Chemical Name | calcium (4S,6S,8S,10E,12R,14R,16E,18S,19S,20R,21S)-19,21-dihydroxy-22-((2R,2'R,5R,5'S)-5'-((R)-1-hydroxyethyl)-2,5'-dimethyloctahydro-[2,2'-bifuran]-5-yl)-4,6,8,12,14,18,20-heptamethyl-11-oxido-9-oxodocosa-10,16-dienoate |
Canonical SMILES | O[C@H](C)[C@@]1(C)O[C@H](CC1)[C@]2(C)O[C@@H](C[C@@H]([C@@H](C)[C@H]([C@H](/C=C/C[C@@H](C)C[C@@H](C)/C([O-])=C\C([C@@H](C)C[C@@H](C)C[C@@H](C)CCC([O-])=O)=O)C)O)O)CC2.[Ca+2] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
钙离子(钙)信号通路在受精中发挥主要作用,精子不仅提供其遗传物质,也使细胞内钙离子浓度升高,这对于完成卵母细胞活化和胚胎发育是必要的。离子霉素是由链霉菌产生的离子载体。工业上,离子霉素通常为游离酸或钙盐。
体外实验:离子霉素是用于研究胞内钙水平的提高和研究跨膜钙转运的工具。它也可以用来刺激胞内产生细胞因子,如干扰素、穿孔素、IL-2和IL-4。这些细胞因子在炎症反应中发挥重要作用[1]。
在体实验:10 μmol/l的离子霉素有效激活卵母细胞的活化和随后的发育效率。二次给予5 μmol/l离子霉素明显改善囊胚发育。在体外或体内小鼠胚胎发育中使用离子霉素无副作用[1]。
临床试验:截至目前,离子霉素仍处于临床前开发阶段。
参考文献:
[1] Heytens E, Soleimani R, Lierman S, De Meester S, Gerris J, Dhont M, Van der Elst J, De Sutter P. ? Effect of ionomycin on oocyte activation and embryo development in mouse. Reprod Biomed Online. 2008 Dec;17(6):764-71.
Cell experiment:[1] | |
Cell lines | Human bladder cancer cell line HT1376 |
Reaction Conditions | 0 ~ 100 μg/ml ionomycin for 0 ~ 96 h incubation |
Applications | Thein vitrogrowth rate of HT1376 cells was suppressed by ionomycin in a dose- and time-dependent manner. After continuous treatment with 10 μg/ml ionomycin, characteristic apoptotic DNA degradation, as well as marked decreases in the ratios of Bcl-2 to Bax mRNA and protein could be observed in HT1376 cells. |
Animal experiment:[1] | |
Animal models | Athymic nude mice bearing HT1376 tumors |
Dosage form | 50 μg of ionomycin in 100 μl PBS Injected intratumorally three times weekly for 4 weeks |
Applications | Intratumoral injection of ionomycin into subcutaneous HT1376 tumors reduced the tumorigenicity in nude mice. Furthermore, thesein vivogrowth-inhibitory effects of ionomycin were significantly enhanced by pretreatment with cisplatin. |
Note | The technical data provided above is for reference only. |
References: 1. Miyake H, Hara I, Yamanaka K, et al. Calcium ionophore, ionomycin inhibits growth of human bladder cancer cells both in vitro and in vivo with alteration of Bcl-2 and Bax expression levels. The Journal of Urology, 1999, 162(3 Pt 1): 916-921. |