IWP 4

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IWP 4

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

686772-17-8

包装与价格：

包装	价格(元)
10mg	电议
50mg	电议

产品介绍

化学性质

Physical Appearance	A crystalline solid
Storage	Store at -20°C
M.Wt	496.62
Cas No.	686772-17-8
Formula	C₂₃H₂₀N₄O₃S₃
Solubility	insoluble in H2O; insoluble in EtOH; insoluble in DMSO
Chemical Name	2-((3-(2-methoxyphenyl)-4-oxo-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)thio)-N-(6-methylbenzo[d]thiazol-2-yl)acetamide
Canonical SMILES	O=C(N(C1=C(OC)C=CC=C1)C(SCC(NC2=NC(C=CC(C)=C3)=C3S2)=O)=N4)C5=C4CCS5
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

IWP 4 is an inhibitor of Wnt production. IWP 4 impairs Wnt pathway activity (IC50 = 25 nM) by inactivating Porcupine, a membrane-bound acyltransferase responsible for attaching a palmitoyl group to Wnt proteins, which is essential to Wnt signaling and Wnt secretion. The Wnt family of signaling proteins is involved in most aspects of embryonic development and postembryonic tissue homeostasis. Hyperactivation of the Wnt/β-catenin pathway induces premature senescence of stem cells and age-related loss of stem cell function. In cancer, hyperactivation of the Wnt/β-catenin pathway often exists along with mutations in other cell growth regulatory genes, which results in aberrant cell growth. Thus, IWP 4 could serve a useful tool for researching tissue regeneration and Wnt ligand-dependent tumors. IWP 4 has been used for inducing cardiomyocyte differentiation from human pluripotent stem cells.

References:

1. Chen B, Dodge ME, Tang W, et al. Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chemical Biology, 2009, 5(2): 100-107.

2. Lian X, Hsiao C, Wilson G, et al. Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(27): E1848-1857.

试验操作

Cell experiment:[1]
Cell lines	L-Wnt-STF cells that exhibit constitutive Wnt pathway activation
Reaction Conditions	5 μM IWP 4 for 24 h incubation
Applications	IWP 4 blocked Wnt-dependent phosphorylation of the low-density lipoprotein receptor-related protein 6 (Lrp6) receptor and the scaffold protein Dishevelled (Dvl), thereby preventing the accumulation of β-catenin.
Note	The technical data provided above is for reference only.
	References: 1. Chen B, Dodge ME, Tang W, et al. Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chemical Biology, 2009, 5(2): 100-107. 2. Lian X, Hsiao C, Wilson G, et al. Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(27): E1848-1857.