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Caspase-6 Colorimetric Assay Kit
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Caspase-6 Colorimetric Assay Kit图片
包装与价格:
包装价格(元)
25 assays电议
100 assays电议
200 assays电议
400 assays电议

产品介绍

组份和存储

ComponentsK2015-25
25 assays
K2015-100
100 assays
K2015-200
200 assays
K2015-400
400 assays
Part Number
Cell Lysis Buffer25 ml100 ml100 ml100 mlK2015-C-1
2 X Reaction Buffer2 ml4 x 2 ml16 ml32 mlK2015-C-2
VEID-pNA (4 mM)125 μl500 μl2 x 0.5 ml2 x 1 mlK2015-C-3
DTT (1 M)100 μl400 μl400 μl400 μlK2015-C-4

Store at -20°C.

资料参考

Cysteine-dependent aspartate-directed proteases (Caspases) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Sequential activation of caspases plays an important role in cell apoptosis. Caspase-6 is a member of the caspase family. Caspase-6 is processed by caspases 7, 8 and 10, and can also undergo self-processing. Caspase-6 acts as a downstream enzyme in the caspase activation cascade and interacts with Caspase 8.

 Caspase-6 Colorimetric Assay Kit provides a convenient and simple way for detecting the VEID-dependent caspase activity. When cleavage of VEID from the labeled substrate VEID-p-nitroaniline (VEID-pNA) by Caspase-6 or related caspases, the free pNA light emission can be quantified by using a microtiter plate reader or a spectrophotometer at 405 or 400 nm. Comparison of the absorbance of free pNA from an apoptotic sample with an uninduced control determines the fold increase in Caspase-6 activity.

Features & Properties

FeaturesFast and convenient Simple one-step procedure; takes 1-2 hours. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-6 activity.
ShippingGel pack
Storage ConditionsStore at -20°C.

概述

Species reactivityMammalian
ApplicationsDetect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Kit componentsCell Lysis Buffer 2X Reaction Buffer VEID-pNA (4 mM) DTT (1 M)