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Glucagon
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Glucagon图片
包装与价格:
包装价格(元)
5mg电议
10mg电议

产品介绍
Glucagon is a 29 amino acid peptide that is secreted from pancreatic α-cells in response to low levels of blood glucose.
PurityGreater than 96.0% as determined by RP-HPLC.
Phycical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
Shipping ConditionShipped at Room temp.
SynonymsGLP1; GLP2; GRPP.
Amino Acid SequenceHis-Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-OH.
SolubilityIt is recommended to reconstitute the lyophilized Glucagon in a sterile 1% HCl solution at a concentration of 0.1-1 mg/ml, which can then be further diluted to other aqueous solutions.
FormulationGlucagon peptide was formulated with no additives.
Introduction

Glucagon is an important hormone involved in carbohydrate metabolism. The hormone is synthesized and secreted from alpha cells (?-cells) of the islets of Langerhans, which are located in the endocrine portion of the pancreas. Glucagon is released when the glucose level in the blood is low (hypoglycemia), causing the liver to convert stored glycogen into glucose and release it into the bloodstream. The action of glucagon is thus opposite to that of insulin, which instructs the body's cells to take in glucose from the blood in times of satiation.Glucagon is beneficial for the culture of some cell types. It has been used in some biochemical regulation studies of glycogenolysis in hepatocytes. It has been also been found to induce DNA replication in primary cultures of adult rat hepatocytes when used in combinations with EGF and Insulin. Glucagon increases the blood glucose concentration by promoting rapid breakdown of liver glycogen, and also acts to relax smooth muscle such as the gastrointestinal tract.

Stability

Glucagon although stable at room temperature for 3 weeks, should be stored desiccated below -18℃ . Upon reconstitution Glucagon should be stored at 4℃ between 2-7 days and for future use below -18℃ .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

产品描述

Glucagon is a 29 amino acid peptide that is secreted from pancreatic α-cells in response to low levels of blood glucose[2]. The best-described effect of glucagon is its action on the liver, where it increases blood glucose through stimulation of gluconeogenesis and glycogenolysis[1]. The non-glycemic effects of glucagon include the regulation of food intake and satiety, lipid homeostasis, insulin secretion and energy expenditure[3].

Glucagon, at concentrations of 0.3 and 3 uM, inhibited the chemotactic response of neutrophils towards all chemoattractants tested[4]. Glucagon may promote insulin secretion in MIN6 cells by reducing the production of cGMP in MIN6 cells and increasing the proliferative activity of MIN6 islet β cell line[8]. Thyroxine and glucagon can significantly reduce the mRNA transcription and protein expression levels of HSPs under high temperature conditions[9].

While using the non-invasive barometric plethysmography,glucagon (1 µg/kg, i.n.) significantly inhibited methacholine-induced bronchospasm in mice[5]. The effects of intraperitoneal glucagon injections on intake of a palatable milk diet were tested in rats maintained with ad lib access to pelleted diet. Injections of 25--800 micrograms/kg glucagon administered at meal onset inhibited meal size by 17--36%, but did not affect the normal postprandial behavioral satiety sequence or elicit any behavioral signs of toxicity[6]. Hepatic portal infusion of 13.6 micrograms glucagon/meal reduced the size of spontaneous meals both early and late in the dark in neurally intact rats, but not in hepatic-vagotomized rats[7].

References:
[1]. Jiang G, Zhang BB. Glucagon and regulation of glucose metabolism. Am J Physiol Endocrinol Metab. 2003 Apr;284(4):E671-8. doi: 10.1152/ajpendo.00492.2002. PMID: 12626323.
[2]. Campbell JE, Drucker DJ. Islet α cells and glucagon--critical regulators of energy homeostasis. Nat Rev Endocrinol. 2015 Jun;11(6):329-38. doi: 10.1038/nrendo.2015.51. Epub 2015 Apr 7. PMID: 25850661.
[3]. Kleinert M, Sachs S,et,al. Glucagon Regulation of Energy Expenditure. Int J Mol Sci. 2019 Oct 30;20(21):5407. doi: 10.3390/ijms20215407. PMID: 31671603; PMCID: PMC6862306.
[4]. Insuela DBR, Ferrero MR, et,al. Glucagon Reduces Neutrophil Migration and Increases Susceptibility to Sepsis in Diabetic Mice. Front Immunol. 2021 Jul 6;12:633540. doi: 10.3389/fimmu.2021.633540. PMID: 34295325; PMCID: PMC8290340.
[5]. Insuela DB, Daleprane JB, et,al. Glucagon induces airway smooth muscle relaxation by nitric oxide and prostaglandin E?. J Endocrinol. 2015 Jun;225(3):205-17. doi: 10.1530/JOE-14-0648. PMID: 26021821.
[6]. Geary N, Smith GP. Pancreatic glucagon and postprandial satiety in the rat. Physiol Behav. 1982 Feb;28(2):313-22. doi: 10.1016/0031-9384(82)90081-6. PMID: 7079345.
[7]. Geary N, Le Sauter J, et,al. Glucagon acts in the liver to control spontaneous meal size in rats. Am J Physiol. 1993 Jan;264(1 Pt 2):R116-22. doi: 10.1152/ajpregu.1993.264.1.R116. PMID: 8430871.
[8].Wu Y Y. The effect of glucagon on insulin secretion in MIN6 cells and the effect of cGMP signaling pathway.2014. MA thesis, Shihezi University.
[9]. Cui Ruilian, et al." Effects of thyroxine and glucagon on heat shock protein expression in Bovine mammary epithelial cells cultured at high temperature in vitro." Chinese Journal of Agriculture 25.06(2010):173-177.