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Spectinomycin(hydrochloride hydrate)
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Spectinomycin(hydrochloride hydrate)图片
CAS NO:22189-32-8
包装与价格：
包装价格(元)
1g电议
5g电议
10g电议

产品介绍

化学性质

StorageStore at -20°C
M.Wt495.4
Cas No.22189-32-8
FormulaC14H24N2O7·2HCl [5H2O]
Solubility≥49.5 mg/mL in H2O; ≥3.61 mg/mL in EtOH with gentle warming and ultrasonic; ≥92 mg/mL in DMSO
Chemical Name(5aR,9aR,10aS)-decahydro-4aR,7S,9S-trihydroxy-2R-methyl-6S,8R-bis(methylamino)-4H-pyrano[3-b][1,4]benzodioxin-4-one, dihydrochloride pentahydrate
Canonical SMILES[H][C@@]12[C@@](C(C[C@@H](C)O2)=O)(O)O[C@]3([H])[C@@H](NC)[C@@H](O)[C@@H](NC)[C@H](O)[C@@]3([H])O1.Cl.Cl.O.O.O.O.O
运输条件蓝冰运输或根据您的需求运输。
一般建议为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Spectinomycin is an aminocyclitol antibiotic produced by S. spectabilis with activity against gram-negative bacterial species. Spectinomycin has been used for the treatment of gonorrhea, where it inhibits microbial protein synthesis by binding to the 30S ribosomal subunit, protects the N‐7 position of E. coli 16S rRNA residue G1064 from methylation by dimethyl sulfate. Binding of spectinomycin at this location is thought to stabilize helix 34, inhibiting the binding of elongation factor G, thereby blocking translocation of peptidyl‐ RNAs from the ribosomal A site to the P site [1].

Spectinomycin selectively inhibited protein synthesis in cells and in extracts of E. coli. Mutations to high-level resistance to this antibiotic map close to the streptomycin locus, and the site of action of spectinomycin, like that of streptomycin, is the 30S ribosomal subunit, as shown by experiments with reconstituted 70S ribosomes containing subunits from sensitive and from resistant ribosomes [2].

References:
[1] Zimmerman J M, Maher L J.  In vivo selection of spectinomycin‐inding RNAs[J]. Nucleic acids research, 2002, 30(24): 5425-5435.
[2] Davies J, Anderson P, Davis B D.  Inhibition of protein synthesis by spectinomycin[J]. Science, 1965, 149(3688): 1096-1098.

试验操作

Cell experiment:[1]

Cell lines

Escherichia coliB

Reaction Conditions

50 μg/mL spectinomycin

Applications

When added to an exponentially growing culture, spectinomycin (50 μg/mL) rapidly and reversibly inhibited growth ofEscherichia coliB. Amino acid incorporation was slowed immediately but RNA synthesis continued.

Animal experiment:[2]

Animal models

Male Sprague-Dawley rats, 200 ~ 225 g

Dosage form

10 mg/kg

Administered intravenously (IV)

Applications

Following IV administration, approximately 55% of the drug was excreted into the urine in unchanged form. Spectinomycin at the dose of 10 mg/kg showed a peak plasma concentration of 37.8 μg/mL and a systemic exposure (area-under the curve AUC0-∞) of 15.7 μg h/mL.

Note

The technical data provided above is for reference only.

References:

1. Davies J, Anderson P, Davis BD. Inhibition of protein synthesis by spectinomycin. Science, 1965, 149(3688): 1096-1098.

2. Madhura DB, Lee R, Meibohm B. Pharmacokinetic profile of spectinomycin in rats. Pharmazie, 2013, 68(8): 675-676.