CFDA-SE

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CFDA-SE

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

150347-59-4

包装与价格：

包装	价格(元)
10mg	电议
25mg	电议

产品介绍

化学性质

Storage	Store at -20°C
M.Wt	557.46
Cas No.	150347-59-4
Formula	C₂₉H₁₉NO₁₁
Solubility	≥37.17 mg/mL in DMSO with ultrasonic; insoluble in EtOH; insoluble in H2O
Chemical Name	3',6'-bis(acetyloxy)-3-oxo-2,5-dioxo-1-pyrrolidinyl ester-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid
Canonical SMILES	CC(OC1=CC2=C(C3(C4=C(C=C(OC(C)=O)C=C4)O2)C5=C(C(O3)=O)C=C(C(ON6C(CCC6=O)=O)=O)C=C5)C=C1)=O.CC(OC7=CC8=C(C9(C%10=C(C(O9)=O)C=CC(C(ON%11C(CCC%11=O)=O)=O)=C%10)C%12=C(C=C(OC(C)=O)C=C%12)O8)C=C7)=O
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

CFDA SE，全称为Carboxyfluorescein diacetate, succinimidyl ester，具有细胞膜通透性，一种广泛用于活细胞示踪或细胞增殖检测的荧光染料。CFDA SE本身不发光，进入活细胞后可以被细胞内的酯酶催化分解成CFSE，CFSE可发强烈的绿色荧光，后者不能穿透细胞膜，能完好的保留在细胞内。CFSE可以偶发性地并不可逆地和细胞内蛋白的Lysine残基或其它氨基发生结合反应，从而标记上这些蛋白。被CFDA SE标记的非分裂细胞的荧光非常稳定，稳定标记的时间可达数个月。

CFDA SE标记细胞的荧光非常均匀和稳定，荧光可平均分配至两个子代细胞中[1]，这样每分裂一次子代细胞的荧光会减弱一半，通过流式细胞仪检测就可以检测出没有分裂的细胞，分裂一次的细胞(1/2的荧光强度)，分离两次的细胞(1/4的荧光强度)，分裂三次的细胞(1/8的荧光强度)以及类似的更多分裂次数的细胞。使用CFDA SE检测可以提供整个细胞群中有多少比例的细胞分裂了1次、2次或更多次数，可以检测分裂多达8次或更多次数的细胞增殖[2]。

目前CFDA SE标记细胞后通常用流式细胞仪进行细胞增殖检测。最常用于淋巴细胞的增殖检测，也可以用于成纤维细胞、NK细胞等其它细胞的增殖检测，甚至还可以用于细菌增殖的检测。CFDA SE标记细胞呈绿色荧光，Ex=494 nm，Em=521 nm。使用流式细胞仪检测时可以采用FL1 detection channel。CFDA SE标记的细胞也可以用荧光显微镜进行观察。CFDA SE标记的细胞可用于体外和体内增殖研究，且都不会使邻近细胞染色[3]。

References:

[1] Azarsiz E, Karaca N, Ergun B, et al. In vitro T lymphocyte proliferation by carboxyfluorescein diacetate succinimidyl ester method is helpful in diagnosing and managing primary immunodeficiencies. J Clin Lab Anal. 2018;32(1):e22216.

[2] Lyons AB, Blake SJ, Doherty KV. Flow cytometric analysis of cell division by dilution of CFSE and related dyes. Curr Protoc Cytom. 2013;Chapter 9:Unit9.11.

[3] Li X, Dancausse H, Grijalva I, et al. Labeling Schwann cells with CFSE-an in vitro and in vivo study. J Neurosci Methods. 2003 May 30;125(1-2):83-91.

试验操作

Cell experiment:[1]
Cell lines	Human erythroleukaemic cell line K562, mouse lymphoma cell line YAC-1, human mammary cancer cell line MCF-7 and human melanoma cell line A375
Reaction Conditions	1, 1.5, 2, 2.5, 5 and 10 μM CFDA-SE
Applications	CFDA-SE at 2.5 μM stained more than 95% of the cells on all cell lines tested, and dose-dependently increased fluorescence intensity of stained cells. The optimal concentration for K562 and YAC-1 was found to be 2.5 μM, while the optimal concentrations for A375 and MCF-7 were found to be 5 μM and 10 μM, respectively. Within the 6 h experiment period, no cytotoxicity related to CFDA-SE was observed.
Animal experiment:[4]
Animal models	C57BL/6 mice, aged 5 ~ 8 weeks
Dosage form	10 μM Injected into thymic lobe
Applications	CFDA-SE, at 80 times the concentration used forin vitrolabeling, was nontoxic and labeled randomly approximately 15% of thymocytes 24 h after injection. The turnover rate of labeled thymic emigrants in the lymph nodes was in the order of 21 days. Thus, CFDA-SE may serve as a powerful tool in relatively long-term migration studies.
Note	The technical data provided above is for reference only.
	References: 1. Wang XQ, Duan XM, Liu LH, et al. Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling. Acta Biochimica et Biophysica Sinica (Shanghai), 2005, 37(6): 379-385. 2. Weston SA, Parish CR. New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy. Journal of Immunological Methods, 1990, 133(1): 87-97. 3. Parish CR, Glidden MH, Quah BJ, et al. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Current Protocols in Immunology, 2009, Chapter 4: Unit4.9. 4. Graziano M, St-Pierre Y, Beauchemin C, et al. The fate of thymocytes labeled in vivo with CFSE. Experimental Cell Research, 1998, 240(1): 75-85.