CAS NO: | 121714-22-5 |
包装 | 价格(元) |
500ug (solution) | 电议 |
1mg (solution) | 电议 |
Physical Appearance | A solution in acetonitrile. To change the solvent, simply evaporate the acetonitrile under a gentle stream of nitrogen and immediately add the solvent of choice. |
Storage | Store at -20°C |
M.Wt | 1129.85 |
Cas No. | 121714-22-5 |
Formula | C51H50Cl2N2O23 |
Solubility | Soluble in DMSO |
Chemical Name | bis(acetoxymethyl) 2,2'-((4-(6-(acetoxymethoxy)-2,7-dichloro-3-oxo-3H-xanthen-9-yl)-2-(2-(2-(bis(2-(acetoxymethoxy)-2-oxoethyl)amino)-5-methylphenoxy)ethoxy)phenyl)azanediyl)diacetate |
Canonical SMILES | ClC1=C(C=C(C(C(C2=CC(OCCOC3=C(C=CC(C)=C3)N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C2)=C4C=C5Cl)=C1)OC4=CC5=O)OCOC(C)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Fluo-3 AM是一种具有细胞渗透性的、高亲和力的 Ca2+指示剂。Fluo-3 AM穿透细胞膜进入活细胞后被细胞内的酯酶剪切形成Fluo-3,从而被滞留在细胞内。Fluo-3以游离配体形式存在时几乎是非荧光性的,但是当它与细胞内钙离子结合后可以产生较强的荧光,荧光会增加60至80倍,最大激发波长为 506nm,最大发射波长为 526nm。可以使用激光共聚焦显微镜或流式细胞仪检测细胞内钙离子浓度的变化[1,2]。
与许多其他Ca2+指示剂(如Fluo-4,Calcium Green-1,Calcium Orange,Oregon Green 488 BAPTA-1和Fura-Red)相比,Fluo-3被证明是最适用的Ca2+指示剂,但是更容易发生光漂白[3]。
References:
[1] June CH, Moore JS. Measurement of intracellular ions by flow cytometry. Curr Protoc Immunol. 2004;Chapter 5:.
[2] Bkaily G, Al-Khoury J, Simon Y, Jacques D. Intracellular Free Calcium Measurement Using Confocal Imaging. Methods Mol Biol.
[3] Thomas D, Tovey SC, Collins TJ, Bootman MD, Berridge MJ, Lipp P. A comparison of fluorescent Ca2+ indicator properties and their use in measuring elementary and global Ca2+ signals. Cell Calcium. 2000;28(4):213–223.