包装 | 价格(元) |
10mM (in 1mL DMSO) | 电议 |
25mg | 电议 |
50mg | 电议 |
500mg | 电议 |
Cell lines | PASMCs |
Preparation method | The solubility of this compound in DMSO is >25.4 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 ℃ for several months. |
Reacting condition | 1 μM |
Applications | In PASMCs, pretreatment with 1 μM Sildenafil Citrate increased the phosphorylation of ERK1/ERK2, the percentage of cells in S phase, as well as cell proliferation, compared with serotonin stimulation alone. Furthermore, pretreatment with 10 μM U0126 for 30 mins prevented Sildenafil Citrate-induced increases in the phosphorylation of ERK1/ERK2, and abolished Sildenafil Citrate-induced cell cycle progression and PASMC proliferation. |
Animal models | Hypercholesterolemic MetS rabbits |
Dosage form | 5 mg/kg/day; p.o. |
Applications | In hypercholesterolemic MetS rabbits, Sildenafil Citrate inhibited the development of endothelial dysfunction and Erectile dysfunction (ED). Moreover, Sildenafil Citrate significantly improved relaxation responses of cavernosal tissues. In addition, compared with the control group, Sildenafil Citrate maintained the in vitro erectile responses of the corporal tissue strips in MetS rabbits. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
产品描述 | Sildenafil Citrate is a potent inhibitor of cGMP-specific phosphodiesterase including PDE5 with IC50 values of 3.6 nM.[1] |