Animal experiment:

Rats: The rats are fasted for 12 h before the experiment. Complanatoside A is formulated in water and a single dose of 30 mg/kg is administered by oral gavage. Blood samples are obtained from the vena orbitalis just prior to dose and at 0.083, 0.167, 0.25, 0.5, 0.75, 1, 2, 3, 5, 7, 10 and 14 h after administration. After centrifugation at 6000g for 10 min, the upper plasma is collected and stored at–20℃ before analysis. The data are calculated by Drug&Statistics software[1].

Complanatoside A is a flavonol glycoside isolated from Astragalus complanatus, and currently it is used as a quality control index for A. complanatus in the 2010 edition of the Chinese Pharmacopoeia.

A simple and sensitive LC-MS/MS method is developed for the determination of complanatoside A in rat plasma over the range of 2.3–575 ng/mL. Complanatoside A is extracted from plasma by a protein precipitation procedure, separated by LC and detected by MS/MS in positive electrospray ionization mode. The lower limit of quantification is established at 2.3 ng/mL. Intra- and inter-day precisions (LLOQ, low-QC, med-QC and high-QC) are less than 7.9%, and accuracies are between 94.0 and 105.1%. Matrix effect is acceptable (97.9–103.0%) and extraction recovery is reproducible (88.5–94.4%). Complanatoside A is stable in the investigated conditions. The method is applied to the pharmacokinetics of complanatoside A in rats[1].

References:
[1]. Li N, et al. Quantification of complanatoside A in rat plasma using LC-MS/MS and its application to a pharmacokinetic study. Biomed Chromatogr. 2016 Jun;30(6):888-93.