| 包装 | 价格(元) |
| 1mg | 电议 |
| 5mg | 电议 |
Coelenterazine 是载水母发光蛋白和海肾荧光素酶的发光酶底物。海肾荧光素酶和底物腔肠素已被用作生物发光共振能量转移 (BRET) 中的生物发光供体，以检测蛋白质-蛋白质相互作用。腔肠素是一种超氧阴离子敏感的化学发光探针，也可用于过氧亚硝酸盐的化学发光检测。
Cell lines | RAW 264.7 cells |
Preparation Method | 5 × 104cRAW 264.7 cells were added to wells of a 96-well black plate in triplicate, cell counts were performed, and native coelenterazine was added to the sample (final coelenterazine concentration 0-100 μmol/l). Plates were imaged using an IVIS low-light imaging system |
Reaction Conditions | final coelenterazine concentration 0-100 μmol/l，60min |
Applications | RAW 264.7 chemiluminescent signal intensity positively correlates with coelenterazine concentration in culture. The production of chemiluminescence by RAW 264.7 cells in the absence of stimulation is consistent with the detection of superoxide anion production during oxidative phosphorylation. |
Animal models | 10-26 weeks thirty-six mice (16 females, 20 males) |
Preparation Method | 450 nl of virus (three sites) were injected into the cerebral cortex of mice, followed by coelenterazine injections at 28-day intervals (minimum = 26 days, maximum time = 74 days). Coelenterazine (dissolved in DMSO) and diluted to a concentration of 2.43 mM in sterile water (1 mg/ml) for cortical injection through a 34-gauge needle for imaging and electrophysiological recordings. |
Dosage form | 0.2μl, 0.4μl and 1μl，the injection rate was 1.25 μL/min |
Applications | Coelenterazine strongly boosted activity in LMO3-expressing mice, photon production was proportional to emission rate, and maximal doses of coelenterazine moderately increased neural activity in naive mice and GLuc cohorts, which itself can be produced at high concentrations Nonspecific excitatory effects. |
| 文献引用 | |
| 产品描述 | Coelenterazine is a substrate of various luciferases such as Renilla luciferase (Rluc) and Gaussia luciferase (Gluc), and is also a cofactor of aequorin. It is widely used and can be used for detection Calcium concentration in living cells, gene reporter assays, BRET (Bioluminescence Resonance Energy Transfer) studies, ELISA, HTS, and chemiluminescence detection of ROS levels in tissues or cells[1]. In vitro, coelenterazine enabled the dynamic monitoring of the RAW 264.7 cell oxidative burst[2].Coelenterazine chemiluminescence did not depend on the activity of cell-derived myeloperoxidase.It is a very sensitive and specific superoxide anion chemiluminescent probe[3]. Coelenterazine is a more reliable probe, although susceptible to storage-dependent high background chemiluminescence, but does not require prereductio[4]. In vivo, there were early, preclinical, changes in the localization and magnitude of coelenterazine chemiluminescent foci[2]. Commercially available coelenterazine（soluble in DMSO） is a sensitive substrate for in vivo bioluminescence imaging of coelenterazine luciferase[5]. References: |
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