AZD5438

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AZD5438

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	602306-29-6
包装:	10mg, 25mg, 50mg

包装与价格：

产品名称

AZD 5438

产品介绍

AZD5438是一种有效的CDK1/2/9抑制剂，IC50为16 nM/6 nM/20 nM。对CDK5/6作用效果稍弱，也抑制GSK3β。

细胞实验
细胞系	MCF-7, MDA-MB-231, HCT-116, HT29, LoVo, SW620, Colo-205 cells line
方法	Measurement of Cell Proliferation AZD5438 was tested against solid tumor cell lines as previously described. Briefly, cells were incubated for 48 h with AZD5438 at a range of concentrations. At the end of incubation, the cells were pulsed with 5-bromo-2′-deoxyuridine (BrdUrd) and the amount of DNA synthesis was measured. The IC50 for inhibition of proliferation was specifically determined independently of cell death. Multiple myeloma cell lines were seeded into 96-well plates in RPMI 1640 supplemented with 10% FCS and glutamine and dosed with AZD5438 for 72 h. Cell growth was measured using AlamarBlue (Invitrogen) and GI50 values were calculated with reference to pretreatment control values.
浓度	0~10 μ M
处理时间	72 h

动物实验
动物模型	human HX147 tumor xenografts in Swiss nude (nu/nu genotype; AstraZeneca) mice
配制	prepared in hydroxy-propyl-methyl-cellulose
剂量	37.5-75 mg/kg once or twice daily for ~3 wk
给药处理	oral gavage

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的K_m系数
	动物 A的K_m系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.6921 mL	13.4604 mL	26.9208 mL
5 mM	0.5384 mL	2.6921 mL	5.3842 mL
10 mM	0.2692 mL	1.346 mL	2.6921 mL