生物活性
NU7441 (KU-57788)是一种高度有效的，选择性DNA-PK抑制剂，IC50为14 nM，也抑制PI3K，IC50为5 μM。NU7441抑制DNA依赖性蛋白激酶 (DNA-PK) 比抑制其他PI3K相关激酶 (PIKK)家族成员选择性高，IC50 为14 nM。 电离辐射诱导或Etoposide诱导 DNA 损伤后，1 μM NU7441提高γH2AX病灶的持久性。NU7441 浓度为 0.5 μM 或 1 μM时，作用于SW620 和 LoVo细胞，显著提高电离辐射，Etoposide, 和 Doxorubicin 诱导的G2-M累积增多。
化学数据
| 分子量 | 413.49 |
| 分子式 | C25H19NO3S |
| CAS号 | 503468-95-9 |
| 纯度 | >99% |
| 溶解性（25°C） | DMSO 10 mg/mL |
| 储存和运输条件 | 固体粉末： -20°C 冷藏长期储存
常温运输及临时存放 |
实验操作 来自于公开的文献，仅供相同实验参考（如实验材料、目的不同，请参考其他文献）
| 细胞实验 |
|---|
| 细胞系 | SW620, LoVo, V3, and V3-YAC cells |
| 方法 | Cytotoxicity and growth inhibition studies.
We exposed exponentially growing cells in six-well plates or 6-cm dishes to etoposide or doxorubicin with or without NU7441 (0.5 or 1.0 μmol/L) for 16 hours. For radiosensitization studies, NU7441 was added to the cells 1 hour before irradiation. V3 and V3-YAC cells were exposed to γ-irradiation (3.1 Gy/min 137Cesium, Gammacell 1000 Elite, Nordion International Ltd., Ottawa, Ontario, Canada). SW620 and LoVo were exposed to X-irradiation (2.9 Gy/min at 230 kV, 10 mA; Gulmay Medical Ltd., Camberly, United Kingdom) due to the equipment available. After irradiation, the cells were incubated with or without NU7441 for a further 16 hours. Cells were then harvested by trypsinization, counted, and seeded into 10-cm diameter Petri dishes at densities varying from 100 to 100,000 per dish in drug-free medium for colony formation. Cell growth inhibition following 5-day continuous exposure to NU7441 was determined in LoVo and SW620 cells grown in 96-well plates as described previously. |
| 浓度 | 0, 2, 4, 6, 8μ M |
| 处理时间 | 5 days |
| 动物实验 |
|---|
| 动物模型 | CD-1 nude mice bearing palpable SW620 colorectal cancer xenografts |
| 配制 | dissolved in 40% PEG 400 in saline |
| 剂量 | 10 mg/kg |
| 给药处理 | i.p. |
不同实验动物依据体表面积的等效剂量转换表（数据来源于FDA指南）
| 小鼠 | 大鼠 | 兔 | 豚鼠 | 仓鼠 | 狗 |
| 重量 (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| 体表面积 (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km系数 | 3 | 6 | 12 | 8 | 5 | 20 |
| 动物 A (mg/kg) = 动物 B (mg/kg) × | 动物 B的Km系数 |
| 动物 A的Km系数 |
例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的Km系数（3），再除以大鼠的Km系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。
储备液配制
以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.4184 mL | 12.0922 mL | 24.1844 mL |
| 5 mM | 0.4837 mL | 2.4184 mL | 4.8369 mL |
| 10 mM | 0.2418 mL | 1.2092 mL | 2.4184 mL |
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