| 包装 | 价格(元) |
| 10mM (in 1mL DMSO) | 电议 |
| 50mg | 电议 |
| 100mg | 电议 |
Cell lines | Human colorectal carcinoma cells (SW480, LoVo and HCT-116) |
Preparation Method | The cells were grown at a concentration of 2×105 cells/ml and then treated with capsaicin at concentrations or time points indicated in figure legends. |
Reaction Conditions | 0, 50 and 100 μM for 0, 1, 2, or 3 days |
Applications | Growth of all three human colorectal cancer cell lines was inhibited by capsaicin treatment in a dose- and time-dependent manner. Although 50-μM capsaicin treatment decreased cell growth to some extent, significantly retarded cell growth was observed in cells treated with 100 μM of capsaicin. |
Animal models | Male Swiss albino mice |
Preparation Method | Group 1 (control) received olive oil throughout the course of the experiment. Group 2 were treated with BP (50 mgkg-1 dissolved in olive oil) orally twice a week (Day 1 and Day 4) for four successive weeks. Group 3 received capsaicin (10 mgkg-1 dissolved in olive oil) intraperitoneally once a week for 14 weeks to assess the cytotoxicity, if any, induced by capsaicin. Group 4 (BP + capsaicin) received BP (as for Group 2) along with capsaicin (10 mg kg-1 dissolved in olive oil) intraperitoneally. Capsaicin treatment was started 1 week before the first dose of BP and continued for 14 weeks |
Dosage form | Intraperitoneal injection, 10 mgkg-1 week-1 for 14 week |
Applications | Capsaicin pretreatment resulted in a free radical quenching effect, thereby significantly preventing the peroxidation of lipids in Group 4 animals. |
| 产品描述 | Capsaicin is a highly selective agonist for the transient receptor potential cation channel, subfamily V, member 1 (TRPV1), a ligand-gated, nonselective cation channel, preferentially expressed on small-diameter sensory neurons[1]. capsaicin inhibits CYP1A2, CYP2C9, and CYP2C19, with IC50 values of 2.1, 2.0, and 3.2 μM, respectively, and inhibits CYP2B6, CYP2D6, CYP3A4, and CYP3A5 with extrapolated IC50 values of 24, 18, 38, and 12 μM, respectively[2]. Capsaicin inhibited Human cervical carcinoma HeLa growth (IC50 ~30 μM) and increased intracellular calcium, with effect blocked by capsazepine[3]. Capsaicin induced Human glioblastoma A172 apoptosis at ≥200 μM, not inhibited by capsazepine or BAPTA/AM 250 μM reduced basal generation of ROS and lipid peroxidation H2O2 reduced apoptosis, while NAC enhanced[4]. Capsaicin reduced Human urothelial cancer RT4 growth (IC50=80 μM) via a TRPV1-dependent process, induced cell cycle arrest in G0/G1 phase Apoptosis occurred[5]. Capsaicin pretreated ICR mice CD-1 mice with 2.5 μmol reduced VC-induced (5.8 mol) and TPA-promoted tumor incidence (by 62%) at 22 weeks (ICR) 1 μmol topical capsaicin 24 and 1 hr before, also significantly inhibited tumors induced by topical BP (0.3 μmol) then promoted with TPA (CD-1)[6]. Gavage with capsaicin significantly elevated phase II enzymes in liver and colon Oral capsaicin at 500 ppm for 4 weeks significantly inhibited ACF formation induced by AOM (20 mg/kg body weight, once a week for 2 weeks) In a 38-week study, 500 ppm capsaicin during the 4-week initiation phase significantly reduced (60%) incidence of colonic adenocarcinoma[7]. References: |
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