L67 (DNA Ligase Inhibitor) 是一种竞争性 DNA 连接酶抑制剂,可有效抑制 DNA 连接酶 I/III(IC50 均为 10 μM)。 L67(DNA 连接酶抑制剂)可通过降低线粒体 DNA 水平和增加线粒体产生的 ROS 水平来引起核 DNA 损伤。 L67 (DNA Ligase Inhibitor) 还激活癌细胞中 Caspase 1 依赖性凋亡通路,可用于癌症研究。
Cas No. | 325970-71-6 |
别名 | DNA Ligase Inhibitor |
化学名 | N-(3,5-dibromo-4-methylphenyl)-glycine, 2-[(2-hydroxy-5-nitrophenyl)methylene]hydrazide |
Canonical SMILES | OC1=CC=C([N+]([O-])=O)C=C1/C=N/NC(CNC2=CC(Br)=C(C)C(Br)=C2)=O |
分子式 | C16H14Br2N4O4 |
分子量 | 486.1 |
溶解度 | DMF: 10 mg/ml,DMSO: 10 mg/ml |
储存条件 | Store at -20°C |
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. |
Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
产品描述 | L67 is a competitive inhibitor of DNA ligases I and III (IC50s = 10 and 10 uM for human DNA ligase I and human ligase IIIβ). It blocks DNA binding (Ki = 10 uM), increasing the cytotoxicity of DNA-damaging agents. It also inhibits mitochondrial DNA ligase IIIα and induces the production of reactive oxygen species (ROS) and apoptosis in cancerous, but not non-malignant, cells. It does not inhibit DNA ligase IV or T4 DNA ligase at concentrations up to 100 uM. |