| 包装 | 价格(元) |
| 10mM (in 1mL DMSO) | 电议 |
| 1mg | 电议 |
| 5mg | 电议 |
| 10mg | 电议 |
Cell lines | RAW 264.7 macrophage cells and Bone marrow derived macrophages(BMDMs) |
Preparation Method | RAW 264.7 macrophage cells (1×105cells/well) and BMDMs (0.5×106cells/well) were cultured in 48-well and 12-well plates with DMEM containing 10% FBS, and then incubated at 37℃ under 5% CO2 in an incubator until they reached 95% confluence. Procyanidin C1 (at concentrations of 31.25 and 62.5μg/ml) was added to each well and incubated at 37℃ for 24h. |
Reaction Conditions | 31.25μg/ml and 62.5μg/ml |
Applications | Procyanidin C1 significantly (P |
Animal models | Male ICR mice weighing 35–40g |
Preparation Method | Male ICR mice were treated with a single oral dose of flavan3-ols（FL）, epicatechin（EC）, procyanidin B2（B2), procyanidin C1(C1), cinnamtannin A2(A2), or pentamer fraction(P5). The animals were sacrificed and blood and brown adipose tissue (BAT) sampled. The plasma catecholamine (CA) levels and BAT uncoupling protein (UCP)-1 mRNA expression were determined. |
Applications | A single dose of 10mg/kg FL significantly increased plasma CA and UCP-1 mRNA levels. B2, procyanidin C1, and A2, but not EC and P5 (all at 1mg/kg), significantly increased plasma adrenaline levels. Plasma noradrenaline was significantly elevated by B2 and A2, but not by EC, procyanidin C1, or P5. UCP-1 mRNA levels were significantly increased by procyanidin C1 and P5. In the dose response study of A2, 10-3mg/kg A2 increased UCP-1 mRNA levels significantly, but not 10-2and 10-1mg/kg A2. In addition, combination treatment with 10-1mg/kg A2 and yohimbine, an α2 adrenalin blocker, remarkably increased UCP-1 mRNA levels. |
| 产品描述 | Procyanidin C1 is a polyphenolic compound, it is found in a variety of vegetables and fruits and has a wide range of biological activities, including antioxidant and anti-inflammatory anticancer roles[1,2] Procyanidin C is a catechin trimer purified from Cinnamomi Cortex and it showed inhibitory activity against TGF-β-induced EMT[3]. Treatment with procyanidin C1 in BMDMs resulted in a significant decrease in prostaglandin E2 and cyclooxygenase-2 levels, as well as the expression of cell surface molecules (CD80, CD86, and MHC class II), which was induced by LPS[1]. procyanidin C1 induced cell cycle arrest at S-phase and activated check point kinases, Chk1 and 2 in both MCF-7 and MDA-MB- 231 cells. At 48 h treatment procyanidin C1 induced DNA damage. In addition, procyanidin C1 decreased the Bcl2 levels and increased the BAX levels in both MCF-7 and MDA-MB- 231cells, which indicate that the procyanidin C1 inhibits breast cancer cell growth by inhibiting proliferation and by inducing apoptosis[2] Procyanidin C1, a polyphenolic component of grape seed extract, increases the healthspan and lifespan of mice through its action on senescent cells[4]. In vivo, zebrafish larvae (AB strain) 3 days post-fertilization were incubated with NAC or procyanidins (C, EC, ECG, B1, B2, B3, B4, B1-G, B2-G, C1) in 300 μM H2O2for 4 days. Different grape seed procyanidins increased the survival of PC12 cells challenged with H2O2, improved the movement behavior disorder of zebrafish caused by H2O2, inhibited the increase of ROS and MDA and the decrease of GSH-Px, CAT, and SOD activities, and up-regulated the Nrf2/ARE pathway. The neuroprotective effects of the procyanidin trimer C1 treatment group were greater than the other treatment groups[5] References: |
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