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5-Hydroxy-2'-deoxyuridine
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
5-Hydroxy-2'-deoxyuridine图片
CAS NO:5168-36-5
包装与价格：
包装价格(元)
10mM (in 1mL Water)电议
1mg电议

产品介绍
5-Hydroxy-2'-deoxyuridine(5-OHdU)是一种2'-Deoxycytidine的主要稳定氧化产物。5-Hydroxy-2'-deoxyuridine可以通过DNA聚合酶在体外掺入DNA。
Cas No.5168-36-5
别名5-OHdU
Canonical SMILESOC[C@@H]1[C@H](C[C@H](N2C(NC(C(O)=C2)=O)=O)O1)O
分子式C9H12N2O6
分子量244.2
溶解度Water: 10 mg/mL (40.95 mM)
储存条件Store at -20℃
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

5-Hydroxy-2'-deoxyuridine (5-OHdU) is a major stable oxidation product of 2'-Deoxycytidine. 5-Hydroxy-2'-deoxyuridine can be incorporated into DNA in vitro by DNA polymerase[1].

To study the specificity of nucleotide incorporation opposite 5-hydroxypyrimidines in template DNA, 18- and 45-member oligodeoxyribonucleotides, containing an internal 2'-deoxy-5-hydroxyuridine (5-OHdU) in two different sequence contexts, were used. Translesion synthesis past 2'-deoxy-5-hydroxyuridine (5-OHdU) in both oligonucleotides occurred, but pauses both opposite, and one nucleotide prior to, the modified base in the template is observed. The specificity of nucleotide incorporation opposite 2'-deoxy-5-hydroxyuridine (5-OHdU) in the template is sequence context dependent. In one sequence context, dA is the principal nucleotide incorporated opposite 2'-deoxy-5-hydroxyuridine (5-OHdU). However in a second sequence context, dC is the predominant base incorporated opposite 2'-deoxy-5-hydroxyuridine (5-OHdU). In that same sequence context, dC is also the predominant nucleotide incorporated opposite 2'-deoxy-5-hydroxyuridine (5-OHdU)[1].

[1]. Purmal AA, et al. Major oxidative products of cytosine, 5-hydroxycytosine and 5-hydroxyuracil, exhibit sequence context-dependent mispairing in vitro. Nucleic Acids Res. 1994 Jan 11;22(1):72-8.