| 包装 | 价格(元) |
| 10mg | 电议 |
| 25mg | 电议 |
| 50mg | 电议 |
| 100mg | 电议 |
Cell lines | Human erythroleukaemic cell line K562, mouse lymphoma cell line YAC-1, human mammary cancer cell line MCF-7 and human melanoma cell line A375 |
Preparation Method | The 5 mM CFDA-SE stock in DMSO was diluted to different concentrations (2 μM, 3 μM, 4 μM, 5 μM, 10 μM and 20 μM) in PBS with a total volume of 1 ml. Cells were added to equal volume of CFDA-SE with different concentrations and incubated at 37 C for 5, 6, 7, 8, 10 and 15 min with agitation. |
Reaction Conditions | 1, 1.5, 2, 2.5, 5 and 10 μM CFDA-SE |
Applications | CFDA-SE at 2.5 μM stained more than 95% of the cells on all cell lines tested, and dose-dependently increased fluorescence intensity of stained cells. The optimal concentration for K562 and YAC-1 was found to be 2.5 μM, while the optimal concentrations for A375 and MCF-7 were found to be 5 μM and 10 μM, respectively. Within the 6 h experiment period, no cytotoxicity related to CFDA-SE was observed. |
Animal models | C57BL/6 mice, aged 5 ~ 8 weeks |
Preparation Method | Injected CFDA-SE into thymic lobe |
Dosage form | The concentration of CFDA-SE is 10 μM |
Applications | CFDA-SE, at 80 times the concentration used for in vitro labeling, was nontoxic and labeled randomly approximately 15% of thymocytes 24 h after injection. The turnover rate of labeled thymic emigrants in the lymph nodes was in the order of 21 days. Thus, CFDA-SE may serve as a powerful tool in relatively long-term migration studies. |
| 文献引用 | |
| 产品描述 | CFDA-SE, full name carboxycein diacetate, Succinimidyl Ester, with cell membrane permeability, is a fluorescent dye CFDA-SE widely used for viable cell tracing or cell proliferation detection[1]. The fluorescence of CFDA-SE -labeled cells was very uniform and stable, and the fluorescence could be evenly distributed to the two progeny cells[4]. CFDA-SE can deliver strong green fluorescence,Ex=494 nm,Em=521nm. It cannot penetrate the cell membrane, can be inside the cell intact retention CFDA-SE can not accidental reversibly and intracellular protein Lysine residues or other amino binding reaction occurs, thus tag on these proteins. In Human erythroleukaemic cell line K562, and other cell lines, although all cells are labeled with relatively high fluorescence intensity by CFDA-SE, the efficiency of labeling is highly variable[3].Electron dense vesicles are seen at the ultrastructural level in labeled cells. Discrete vesicular labeling can also be observed in whole cell mounts viewed with fluorescence microscopy. Whole cells retain good label for 6 weeks. CFDA-SE labeling is relatively easy, nontoxic to cells and nonradiocactive[2]. In vitro lymphocyte cell proliferation analysis by CFDA-SE method is a reliable and practical choice for the assessment of mitogenic T lymphocyte responses in yet unclassified PID patients for targeting further genetical analyses[6].When NM is detected by flow cytometry, FL1 detection channel CFDA-SE -labeled cells can be adopted, and fluorescence microscopy can also be used to observe CFDA-SE -labeled cells without staining adjacent cells[8]. CFDA-SE has been reported to be effective in labeling CD34+ cells, CD34+ cells isolated from fetal liver or thymus were labeled with CFDA-SE and were injected into a human thymus grafted subcutaneously in the RAG-2 / IL-2Rγ / mice. One to 4 weeks later the CFDA-SE label was found not only in T cells but also in CD123+/high CD4+CD45RA+ pDC2, indicating that the CD34+ cells can develop into pDC2 within a thymus. In addition to pDC2, CFDA-SE -labeled dendritic cells with a mature phenotype, determined by the cell surface markers CD11c, CD83, and CD80, were found in the injected human thymus graft. pDC2 was not found in the periphery of mice carrying a human thymic graft, indicating that the intrathymic pDC2 failed to emigrate from the thymus[5]. In C57BL/6 mice，CFDA-SE, at 80 times the concentration used for in vitro labeling, was nontoxic and labeled randomly approximately 15% of thymocytes 24 h after injection. The turnover rate of labeled thymic emigrants in the lymph nodes was in the order of 21 days. Thus, CFDA-SE may serve as a powerful tool in relatively long-term migration studies[7]. References: |
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