| 包装 | 价格(元) |
| 500μg | 电议 |
| 1mg | 电议 |
Cell lines | EPC cells |
Preparation Method | Uptake kinetics of EPC cells loaded with 5 μM calcein AM seeded at 1x105cells well-1and cultured at 15 ℃. Calcein AM uptake was measured as fluorescence intensity (FI). |
Reaction Conditions | 5 μM at 15 ℃, 1-8h |
Applications | Uptake kinetics showed that for EPC cells seeded at a density of 1x105cells well-1calcein AM labelling increased throughout the 7 h tested. |
| 文献引用 | |
| 产品描述 | Calcein-AM is a highly lipophilic vital dye that rapidly enters viable cells. It is converted by intracellular esterases to calcein that produces an intense green (530-nm) signal, and is retained by cells with intact plasma membrane.[1] In vitro experiment it shown that calcein-AM assay used to assess human RBC viability after incubation (37℃ for 3 and 20 h) in the presence of Ca2+ (2.5 mM) and ionophore A 23187 (0.5 μM).[1]0.05 μM was the optimal concentration of CAM (Calcein-AM) for staining effector cells by testing 0.05, 0.1, 0.2, and 0.4 μM. Using 0.05 μM CAM to stain the PBMCs and expanded NK cells from three normal volunteers, the results demonstrated that there is no significant decrease in cytotoxicity and CAM staining had no significant effect on human NK cell activity in PBMCs or in expanded NK cells.[2]In vitro, 50μm calcein AM's fluorescent signal of 1 x lo5 lymphocytes was close to the saturation level, while the signal emitted by lymphocytes labeled with 20μm calcein AM was only slightly lower.[3]Calcein-AM has cytotoxic activity against human tumor cell lines (such as the human lymphoma U-937-GTB) at low concentrations (2.5 ug/ml).[4]In vitro experiment it demonstrated that in the mixed macrophages and THP-1 cells (5x105 cells/ml), Calcein-AM (2 μM)/propidium Iodide (PI) (4.5 μM) staining assay Calcein-AM/PI double staining was used to quantify the number of living and dead cells as a cell death assay.[5]In addition, The cells in OA chondrocytes were seeded in 24-well plates (2 × 104 cells/well), cultured for 4 h, the cells were treated with 5 μL Calcein-AM (2 μM) and 5 μL PI (2 μM) at 37℃ in conditions void of light for 30 min, and then analyzed under a fluorescence microscope.[6] References: |
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