Cell lines

human endometrial stromal cells

Preparation method

The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

7 days, 100 nM

Applications

Etonogestrel is the active metabolite of prodrug desogestrel. Etonogestrel activates GR signaling and induces FKBP51 mRNA and protein expression in HESCs.

Animal models

Female C57BL/6 mice aged 8–12 weeks

Dosage form

Ovariectomized mice received three daily s.c. injections of 100 ng 17β-estradiol. Then mice were rested for 3 days before insertion of one-fourth of a standard Implanon rod containing etonogestrel subcutaneously into the back of the animals. Examine the effect of long-term exposure (up to 45 days) to etonogestrel on endometrium.

Application

Large blood vessels developed in decidualized horns. Disturbance of tissue integrity occurred at 5d with substantial stromal breakdown at 10d. This progress proceeded until 25d when re-epithelialization was initiated. At 45d, the tissue was recovered to its pre-decidualized state but with plentiful tortuosity of the luminal epithelium.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.