Tubacin

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Tubacin

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	537049-40-4
包装:	2mg, 5mg, 10mg

包装与价格：

产品名称

Tubulin Acetylation Inducer

产品介绍

Tubacin是一种高效的，选择性的，可逆的，细胞通透性的HDAC6抑制剂，IC50为4 nM。

细胞实验
细胞系	ALL cells and normal T lymphocytes
方法	Growth inhibition assay The inhibitory effect of tubacin on ALL cells and normal T lymphocytes was assessed by colorimetric assay, 3-(4,5-dimethylthiazol-e-yl)- 2,5-diphenyl tetrazolium bromide (MTT; Sigma). Only active mitochondria dehydrogenase enzymes can perform cleavage of a tetrazolium ring, and therefore the amount of purple formazan generated by cells with a drug was compared with the amount of formazan produced from non-treated cells. Absorbance readings at a wavelength of 550 nm were taken on a spectrophotometer (Titertek Multiskan MCC Type 341). Cells from 72 h cultures were incubated with 10 μL of 5 mg/mL MTT for 2 h, followed by 100 μL isopropanol that contained 0.1 N HCl. Normal T lymphocytes were incubated with MTT for 30–37 min. All experiments were performed in triplicate.
浓度	0, 0.5, 1, 1.5, 2, 2.5μM
处理时间	72 h

动物实验
动物模型	Xenograft system of primary leukemia samples
配制	saline
剂量	50 mg/kg/day
给药处理	intraperitoneally

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的K_m系数
	动物 A的K_m系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.3853 mL	6.9266 mL	13.8531 mL
5 mM	0.2771 mL	1.3853 mL	2.7706 mL
10 mM	0.1385 mL	0.6927 mL	1.3853 mL