MF63

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MF63

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	892549-43-8
包装:	2mg, 5mg, 10mg, 50mg

包装与价格：

产品介绍

MF63是一种选择性的mPGES-1抑制剂，猪和人PGES-1酶的IC50分别为0.9 nM和1.3 nM 。

细胞实验
细胞系	A549 cells
方法	A549 cells were seeded in 96-well dishes at a density of 25,000 cells/well and incubated for 20 h in RPMI 1640 medium supplemented with 2% fetal bovine serum (FBS). The cells were then incubated with 10 ng/ml IL-1 for 24 h in the presence of a vehicle or inhibitor from a 100-fold concentrated DMSO stock solution in a medium containing 2 or 50% FBS. Concentrations of PGE2 and PGF2 in the cell-free medium were measured by EIA (Assay Designs Inc.). IC50 values were derived from eight-point titrations.
浓度	0.01~100 μ M
处理时间	24 h

动物实验
动物模型	mPGES-1 Knock-In Mice
配制	a suspension in 0.5% methylcellulose
剂量	100 mg/kg
给药处理	oral gavage

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的K_m系数
	动物 A的K_m系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.6398 mL	13.1992 mL	26.3985 mL
5 mM	0.528 mL	2.6398 mL	5.2797 mL
10 mM	0.264 mL	1.3199 mL	2.6398 mL