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SSE15206
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
SSE15206图片
CAS NO:1370046-40-4
包装与价格:
包装价格(元)
10mM (in 1mL DMSO)电议
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议

产品介绍
SSE15206是一种微管(microtubule)聚合抑制剂,其在HCT116细胞SRB增殖实验中的GI50值为197nM。
Cas No.1370046-40-4
Canonical SMILESCOC1=C(OC)C(OC)=CC(C2N(C(N)=S)N=C(C3=CC=CC=C3)C2)=C1
分子式C19H21N3O3S
分子量371.45
溶解度DMSO : 150 mg/mL (403.82 mM);Water :< 0.1 mg/mL (insoluble)
储存条件Store at -20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

SSE15206 is a microtubule polymerization inhibitor, with a GI50 of 197 nM in a SRB proliferation assay in HCT116 cells.

Treatment of cells with SSE15206 result in rounding up of cells which prompt us to look for induction of mitotic arrest using phosphorylation of histone H3 (S10) and MPM2 as mitotic markers. Similarly, treatment with SSE15206 for 8 and 24 hours increase mitotic index compared to the DMSO control. The increase in the mitotic index is quantified and found to be statistically significant at both concentrations and time points. Activation of mitotic kinases, Aurora A, Aurora B and MPS1 in A549 cells accompanied mitotic arrest following SSE15206 treatment. Treatment with 1.25 μM and 2.5 μM concentrations of SSE15206 result in the failure of microtubules to repolymerize at 37 °C following incubation on ice, similar to the positive control nocodazole[1].

[1]. Manzoor S, et al. Identification and characterization of SSE15206, a microtubule depolymerizing agent that overcomes multidrug resistance. Sci Rep. 2018 Feb 19;8(1):3305.