GDC-0980

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GDC-0980

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	1032754-93-0
包装:	5mg, 10mg, 50mg, 100mg

包装与价格：

产品名称

RG7422

产品介绍

GDC-0980 (RG7422)是一种有效的，I型PI3K抑制剂，作用于PI3Kα/β/δ/γ，IC50分别为5 nM/27 nM/7 nM/14 nM，也抑制mTOR，K_i为17 nM，高选择性作用于其他PIKK家族激酶。

细胞实验
细胞系	NSCLS cell lines, colon cancer cell lines and etc.
方法	Cell viability assays Three hundred and eighty-four–well plates were seeded with 2,000 cells/well in a volume of 54 μL per well followed by incubation at 37°C under 5% CO2 overnight (~16 hours). Compounds were diluted in dimethyl sulfoxide to generate the desired stock concentrations then added in a volume of 6 μL per well. All treatments were tested in quadruplicate. After 4 days incubation, relative numbers of viable cells were estimated using CellTiter-Glo (Promega) and total luminescence was measured on a Wallac Multilabel Reader (PerkinElmer). The concentration of drug resulting in 50% inhibition of cell viability (IC50) or 50% maximal effective concentration (EC50) was determined using Prism software (GraphPad). For cell lines that failed to achieve an IC50 the highest concentration tested (20 μmol/L) is listed.
浓度	0~5μM
处理时间	4 days

动物实验
动物模型	MCF7-neo/HER2 tumor-bearing mice
配制	0.5% methylcellulose with 0.2% Tween-80
剂量	7.5 mg/kg
给药处理	oral gavage

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的K_m系数
	动物 A的K_m系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.0056 mL	10.0281 mL	20.0562 mL
5 mM	0.4011 mL	2.0056 mL	4.0112 mL
10 mM	0.2006 mL	1.0028 mL	2.0056 mL