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FlAsH-EDT2
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
FlAsH-EDT2图片
CAS NO:212118-77-9
包装与价格：
包装价格(元)
1mg电议
5mg电议
10mg电议
25mg电议

产品介绍
FlasH-EDT2 是一种蛋白质标记试剂。
Cas No.212118-77-9
别名荧光含砷螺旋粘结剂/卢米奥绿色,Fluorescein Arsenical Helix Binder, Lumio Green
化学名4',5'-bis(1,3,2-dithiarsolan-2-yl)-3',6'-dihydroxy-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
Canonical SMILESOC1=C([As]2SCCS2)C3=C(C4(C(C=CC=C5)=C5C(O4)=O)C6=CC=C(O)C([As]7SCCS7)=C6O3)C=C1
分子式C24H18As2O5S4
分子量664.5
溶解度DMF: 15 mg/ml,DMSO: 20 mg/ml,DMSO:PBS (pH 7.2) (1:10): 0.09 mg/ml
储存条件Store at -20°C,protect from light
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

FlAsH-EDT2 is a pro-fluorescent, membrane-permeable biarsenical compound that binds covalently to a tetracysteine sequence (CCPGCC), which is engineered into target proteins.[1],[2] It binds proteins that have the CCPGCC tag almost immediately after translation.[2] FlAsH-EDT2 is commonly used to study protein trafficking, folding, and interactions in living cells or cell lysates.[2],[3],[4] This green-emitting fluorophore is excited at 508 nm, with emission at 528 nm.[4]

Reference:
[1]. Griffin, B.A., Adams, S.R., and Tsien, R.Y. Specific covalent labeling of recombinant protein molecules inside live cells. Science 287(5374), 269-272 (1998).
[2]. Rudner, L., Nydegger, S., Coren.L.V., et al. Dynamic fluorescent imaging of human immunodeficiency virus type 1 gag in live cells by biarsenical labeling. J.Virol. 79(7), 4055-4065 (2005).
[3]. Luedtke, N.W., Dexter, R.J., Fried, D.B., et al. Surveying polypeptide and protein domain conformation and association with FlAsH and ReAsH. Nat.Chem.Biol. 3(12), 779-784 (2007).
[4]. Perdios, L., Bunney.T.D., Warren, S.C., et al. Time-resolved FRET reports FGFR1 dimerization and formation of a complex with its effector PLCγ1. Adv.Biol.Regul. 60, 6-13 (2016).