Cell experiment:

MDA-MB-231 and C2C12 cells are treated with 50μM of SIRT5 inhibitor MC3482 for 24 h. Ammonia levels are measured in the culture medium every other day[1].

MC3482 is a specific sirtuin5 (SIRT5) inhibitor.

MC3482 inhibits sirtuin5. 50 μM MC3482 inhibits SIRT5 desuccinylating activity without affecting SIRT5 intracellular expression levels. Autophagy and mitophagy increase in SIRT5-silenced cells and in WT cells treated with MC3482 and decrease in SIRT5-overexpressing cells[1].

[1]. Polletta L, et al. SIRT5 regulation of ammonia-induced autophagy and mitophagy. Autophagy. 2015;11(2):253-70.