16F16 is a protein disulfide isomerase (PDI) inhibitor.¹It inhibits PDI reductase activity in an enzyme assay when used at concentrations ranging from 1 to 100 µg/ml.¹16F16 reduces PC12 cell apoptosis induced by the misfolded huntingtin protein HTT^Q103. It suppresses PDI-dependent mitochondrial outer membrane permeabilization (MOMP) in isolated PC12 cell mitochondria. 16F16 (2, 3, 4, and 10 µM) reduces HTT^N90Q73mutant huntingtin-induced medium spinal neuron death and MOMP in rat corticostriatal slices. It also reduces pyramidal neuron death induced by amyloid-β precursor protein (APP) in rat corticostriatal slices.

1.Hoffstrom, B.G., Kaplan, A., Letso, R., et al.Inhibitors of protein disulfide isomerase suppress apoptosis induced by misfolded proteinsNat. Chem. Biol.6(12)900-906(2010)