5-FAM (5-Carboxyfluorescein) (5-Carboxyfluorescein) 是一种绿色荧光试剂，用于原位标记肽、蛋白质和核苷酸。
| Cas No. | 76823-03-5 |
| 别名 | 5-羧基荧光素; 5-Carboxyfluorescein |
| Canonical SMILES | O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=C(C(O)=O)C=C5 |
| 分子式 | C21H12O7 |
| 分子量 | 376.32 |
| 溶解度 | DMSO : ≥ 31 mg/mL (82.38 mM) |
| 储存条件 | Store at -20°C |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. |
| Shipping Condition | Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request |
| 产品描述 | The single isomer, 5-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser. |
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