Animal experiment:

Horses[1]Seven female and nine male horses are assigned at random to two groups (G1 and G2) submitted to different treatments after the induction of acute tendinitis. Each group consists of eight animals injected with type 1 collagenase, 1 mL, 2.5 mg/mL, in the middle metacarpal third into the superficial digital flexor tendons of both forelimbs. Twenty days after the injection of collagenase, one of the limbs (G1A/G2A) receives an injection of 3-Aminopropionitrile fumarate (2:1), 3 mL, 0.8 mg/mL, and the other limb (G1B) receives the same volume of buffered saline as control. Group one was left in box rest, and group two was submitted to controlled exercise during the experiment[1].

3-Aminopropionitrile fumarate (2:1) is a lathyrogen which inhibits crosslinking of collagen.

Twenty days after the induction of tendinitis, intralesional treatment with 3-Aminopropionitrile fumarate (2:1) (BAPN-F) is performed and the contralateral limbs receive saline. A biopsy is obtained and gross and histopathological analysis is performed on the 150 th day of the experiment. The collagen fibrillar alignment pattern in the healing area is better in the 3-Aminopropionitrile fumarate (2:1) group submitted to controlled exercise than in the other group, as observed by sonographic and histopathologic examination. The present results indicate that 3-Aminopropionitrile fumarate (2:1) in combination with controlled loading improved scar remodeling and tendon wound collagen maturation[1].

[1]. A.L.G. Alves, et al. Effects of beta-aminopropionitrile fumarate and exercise on equine tendon healing: gross and histological aspects. Journal of Equine Veterinary Science, 2001, 21 (7) :335-340.