包装 | 价格(元) |
10mM (in 1mL DMSO) | 电议 |
5mg | 电议 |
10mg | 电议 |
25mg | 电议 |
50mg | 电议 |
Cell lines | meningioma lines (Ben-Men1, IOMM-Lee, HBL-52) |
Preparation method | The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. |
Reaction Conditions | 24 h; 100 μM/mL |
Applications | Cilengitide(1, 10, and 100 μM/mL) was added to IOMM-Lee,HBL52, and Ben-Men1 cultures. Morphologic changes were monitored over 24 hours. In all three meningioma lines, cells strated to round up and detach from the flask in a concentration-dependent manner, showing that cilengitide decreases cell adhesion. Quantification of cell viability after 24 hours, cilengitide treatment showed in all three cell lines a highly significant dose-dependent but rather mild decline of viable cells. |
Animal models | 8- to 10-week-old Swiss Nude mice |
Dosage form | 75 mg/kg; intraperitoneal injection |
Applications | We intended to test a daily dosage of cilengitide (75 mg/kg) as a monotherapy or combined with irradiation in the orthotopic mouse model. A significant reduction of tongue-like brain invasion (P≤0.01) could be observed in tumors of mice treated with either cilengitide alone (35% decrease) or with cilengitide and irradiation (35.5% decrease). |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
产品描述 | Cilengitide is a cyclic RGD pentapeptide [Arg-Gly-Asp-DPhe-(NMeVal)], and a potent αvβ3 and αvβ5 integrin inhibitor to block integrin-mediated adhesion and migration. It can directly bind αvβ3 integrin. Its IC50 is 250 nM as an αvβ3 inhibitor [1] [2] [3] [4]. |