| 包装 | 价格(元) |
| 10mM (in 1mL DMSO) | 电议 |
| 1mg | 电议 |
| 5mg | 电议 |
| 10mg | 电议 |
Cell lines | THP-1 cells |
Preparation Method | THP-1 cells were plated at 4 × 106cells per ml in 6- or 24-well plates for performing the caspase 3 fluorometric assay or in 8-well chamber slides for the TUNEL assay.Live or dead microsporidia spores were added to wells at a parasite to host cell ratio of 3:1. Apoptosis was experimentally induced by treatment with 1 uM of staurosporine after 4 h. |
Reaction Conditions | 1 uM; 4h |
Applications | Macrophages incubated with live E. cuniculi and induced by staurosporine exhibited significantly fewer TUNEL-positive cells compared to macrophages incubated without microsporidia and induced with staurosporine. |
Animal models | female immunocompromised mice, Nu/J-Foxn1 nu/nu |
Preparation Method | When tumors reached a volume of - 65 mm3, mice were randomized into one of four treatment groups: placebo, staurosporine, lapatinib, or combined staurosporine and lapatinib. For the staurosporine single and combination treatment groups, mice received 3 mg/kg staurosporine via oral gavage twice a week. For the lapatinib single and combination treatment groups, mice received 50 mg/kg lapatinib via oral gavage twice a week. Tumor measurements were taken twice weekly on the days of treatment./p> |
Dosage form | 3 mg/kg; p.o. |
Applications | Staurosporine by itself showed no effects on tumor growth, likely due to the fact that we used a low dose of staurosporine (3 mg/kg). However, the combination of 3 mg/kg staurosporine and 50 mg/kg lapatinib impaired tumor growth in a statistically significant manner. |
| 文献引用 | |
| 产品描述 | Staurosporin, a small kinase inhibitor, is an alkaloid derived from the bacterium Streptomyces staurosporeus.[1]Staurosporine can block the ATP-binding site of the enzimes and induce apoptosis by activation of caspase-3 in higher eukaryotes.[2] In vitro experiment it shown that treatment with 50 nM of Staurosporin, there is a single-cell migration of breast carcinoma cells on plastic, fibronectin, or laminin surfaces.[1]Staurosporine killed Acanthamoeba trophozoites in a dose dependent way with IC50 and IC90 values of 0.265 ± 0.057 and 1.27 ± 0,007 μg/mL, respectively.[2]In vitro, treatment with a low concentration (10(-7) M) of staurosporine in cultured rat astrocytes, there is a significantly increased proportion of early apoptotic cells after regeneration in a staurosporine free medium. However, treatment with a higher (10(-6) M) concentration of staurosporine, there is further obviously increased necroptosis after regeneration in a staurosporine free medium.[3]In vitro efficacy test it indicated that 1 μM STS was able to activate the autophagy pathway in SH-SY5Y cells.[4]In addition, treatment with 5 to 50 μM of staurosporine, conidial cell viability decreased in a concentration-dependent manner, suggesting that staurosporine has potent antifungal activity against N. crassa conidia.[5] In vivo efficacy study it demonstrated that mice were treated with 3 mg/kg staurosporine via oral gavage twice a week has no effects on tumor growth. But mice were treated with the combination of 3 mg/kg staurosporine and 50 mg/kg lapatinib inhibited the tumor growth obviously.[6] References: |
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